Brück I, Anderson G A, Hyland J H
Department of Clinical Studies, Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark.
Theriogenology. 1997 Jan 15;47(2):441-56. doi: 10.1016/s0093-691x(97)00003-4.
The influence of different maternal plasma progesterone concentrations on embryonic glucose metabolism was studied. Uterine flushes were obtained after treating ovariectomized mares (n = 3) with 0 (control), 100 or 200 mg progesterone daily for 7 d. A group of progesterone-induced proteins (PIP) of Mr approximately 20,000 were identified in flushes from progesterone treatments by SDS-PAGE but were not observed in control flushes. Progesterone-induced proteins were removed from half the pooled flush in each treatment group by Sepharose blue CL-6B. In a 3 x 2 factorial (progesterone treatments, progesterone-induced proteins) experiment, 6 groups of Day 7 equine embryos (n = 6 per group) were incubated in culture media (MEM:DPBS; 1:3) containing radioactively-labeled glucose. Contributions of the Embden-Meyerhof pathway (EMP) and the pentose-phosphate pathway (PPP) to the total metabolism of glucose in early equine embryos were assessed separately. In the 200 mg progesterone treatment group, the presence of progesterone-induced proteins in the culture medium resulted in a 4-to 5-fold increase in the activities of the Embden-Meyerhof pathway and the pentose-phosphate pathway. These results lead to the following conclusions: 1)Addition of progesterone-induced uterine proteins from mares with high levels of circulating progesterone enhance the metabolic activities of the Embden-Meyerhof pathway and the pentose-phosphate pathway in Day 7.5 equine embryos in culture. 2)Uterine secretion of progesterone-induced proteins which is quantitatively and/or qualitatively adequate to modify embryonic glucose metabolism in vitro is dependent on a minimal concentration of maternal plasma progesterone.
研究了不同母体血浆孕酮浓度对胚胎葡萄糖代谢的影响。对去卵巢母马（n = 3）每日分别给予0（对照）、100或200 mg孕酮，持续7天，之后获取子宫冲洗液。通过SDS-PAGE在孕酮处理组的冲洗液中鉴定出一组分子量约为20,000的孕酮诱导蛋白（PIP），而在对照冲洗液中未观察到。通过琼脂糖蓝CL-6B从每个处理组一半的合并冲洗液中去除孕酮诱导蛋白。在一个3×2析因实验（孕酮处理、孕酮诱导蛋白）中，将6组第7天的马胚胎（每组n = 6）培养于含有放射性标记葡萄糖的培养基（MEM:DPBS；1:3）中。分别评估了糖酵解途径（EMP）和磷酸戊糖途径（PPP）对早期马胚胎葡萄糖总代谢的贡献。在200 mg孕酮处理组中，培养基中孕酮诱导蛋白的存在导致糖酵解途径和磷酸戊糖途径的活性增加了4至5倍。这些结果得出以下结论：1）添加来自循环孕酮水平高的母马的孕酮诱导子宫蛋白可增强培养的第7.5天马胚胎中糖酵解途径和磷酸戊糖途径的代谢活性。2）子宫分泌的孕酮诱导蛋白在数量和/或质量上足以在体外改变胚胎葡萄糖代谢，这取决于母体血浆孕酮的最低浓度。
