Brück I, Hyland J H
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
J Reprod Fertil Suppl. 1991;44:419-25.
The contributions of 2 biochemical pathways to the total metabolism of glucose (the Embden-Meyerhof pathway [EMP] and the pentose phosphate pathway [PPP]), were assessed for equine embryos recovered on Day 4.5, 7.5 and 11.5 post ovulation. At all developmental stages studied, glucose was metabolized through both pathways. Through the EMP, the amounts of glucose metabolized per nl embryo volume per hour were 4.0, 9.9 and 3.1 pmol, whereas via the PPP, amounts were 0.9, 1.7 and 0.07 pmol for Day-4.5, -7.5 and -11.5 embryos, respectively. The ratio of EMP:PPP with age was 9.7 for Day -4.5 embryos, 7.6 for Day-7.5 embryos and 90.2 for Day -11.5 embryos. Two of the 6 unfertilized ova (UFO) exhibited metabolism through both pathways.
对排卵后第4.5、7.5和11.5天回收的马胚胎，评估了两条生化途径（糖酵解途径[EMP]和磷酸戊糖途径[PPP]）对葡萄糖总代谢的贡献。在所有研究的发育阶段，葡萄糖都是通过这两条途径代谢的。通过EMP途径，每小时每纳升胚胎体积代谢的葡萄糖量分别为4.0、9.9和3.1皮摩尔，而通过PPP途径，第4.5、7.5和11.5天胚胎的代谢量分别为0.9、1.7和0.07皮摩尔。EMP与PPP的比率随胚胎年龄变化，第4.5天胚胎为9.7，第7.5天胚胎为7.6，第11.5天胚胎为90.2。6个未受精卵（UFO）中有2个表现出通过这两条途径进行代谢。
