Derecka K, Ziecik A J
Division of Endocrinology and Pathology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.
Theriogenology. 1997 Jul 1;48(1):51-62. doi: 10.1016/S0093-691X(97)00201-X.
The receptors for LH, located in the gonadal tissues of the male and female, mediate stimulation of ovarian and testicular steroidogenesis by the pituitary. However, it is known that several animal and human nongonadal reproductive tissues also contain functional LH receptors. This study was undertaken to compare expression of LH/hCG receptor gene expression in gonadal and nongonadal reproductive tissue of the male and female fetal and neonatal pigs. We used reverse transcription-polymerase chain reaction (RT-PCR) to examine the onset of LH/hCG receptor gene expression in both gonadal and nongonadal tissues during ontogeny of the pig. According to our results the expression of LH/hCG receptor gene as measured by the presence in its extracellular domain starts between 30 and 40 d post coitum in the testis and between 40 and 48 d post coitum in the ovary. The expression of extragonadal LH/hCG receptor gene in the uterus occurred between Days 48 and 103 post coitum. When detected, the extracellular domain of LH/hCG receptor gene was expressed permanently both in gonadal and nongonadal porcine reproductive tissues. We also showed that LH/hCG receptors are expressed in the pig epididymis on Day 1 of neonatal life.
促黄体生成素（LH）的受体位于雄性和雌性的性腺组织中，介导垂体对卵巢和睾丸类固醇生成的刺激作用。然而，已知几种动物和人类的非性腺生殖组织也含有功能性LH受体。本研究旨在比较雄性和雌性胎儿及新生仔猪性腺和非性腺生殖组织中LH/hCG受体基因的表达情况。我们使用逆转录-聚合酶链反应（RT-PCR）来检测猪个体发育过程中性腺和非性腺组织中LH/hCG受体基因表达的起始情况。根据我们的结果，通过其细胞外结构域的存在来衡量，LH/hCG受体基因在睾丸中于交配后30至40天开始表达，在卵巢中于交配后40至48天开始表达。子宫中非性腺LH/hCG受体基因的表达发生在交配后48至103天之间。当被检测到时，LH/hCG受体基因的细胞外结构域在猪的性腺和非性腺生殖组织中均持续表达。我们还表明，新生仔猪出生第一天时，附睾中表达LH/hCG受体。
