Dreanno C, Suquet M, Quemener L, Cosson J, Fierville F, Normant Y, Billard R
Laboratoire d'Ichtyologie, Muséum National d'Histoire Naturelle, 75231 Paris, France.
Theriogenology. 1997 Sep;48(4):589-603. doi: 10.1016/s0093-691x(97)00276-8.
The aim of this study was to develop a method for cryopreserving turbot semen and to compare sperm motility characteristics, metabolic status and fertilization capacity of frozenthawed and fresh semen. The best results were obtained when spermatozoa were diluted at a 1:2 ratio with a modified Mounib extender, supplemented with 10% BSA and 10% DMSO. For freezing sperm samples, straws were placed at 6.5 cm above the surface of liquid nitrogen (LN) and plunged in LN. The straws were thawed in water bath at 30 degrees C for 5 sec. Use of this simple method resulted in a 60 to 80% reactivation rate of the thawed spermatozoa. Although the percentage of motile spermatozoa in the frozen-thawed semen samples was significantly lower than in fresh semen, spermatozoa velocity and respiratory rate remained unchanged. The process of cryopreservation significantly decreased intracellular ATP content. The fertilization rate of frozen-thawed spermatozoa was significantly lower than that of fresh spermatozoa, but it increased with sperm concentration.
本研究的目的是开发一种冷冻保存大菱鲆精液的方法，并比较冻融精液与新鲜精液的精子活力特征、代谢状态和受精能力。当精子以1:2的比例用改良的穆尼布稀释液稀释，并添加10%的牛血清白蛋白和10%的二甲亚砜时，可获得最佳结果。对于冷冻精子样本，将细管放置在液氮表面上方6.5厘米处，然后投入液氮中。细管在30℃的水浴中解冻5秒。使用这种简单方法可使解冻后的精子再激活率达到60%至80%。虽然冻融精液样本中活动精子的百分比明显低于新鲜精液，但精子速度和呼吸速率保持不变。冷冻保存过程显著降低了细胞内ATP含量。冻融精子的受精率明显低于新鲜精子，但随精子浓度的增加而提高。
