Oh Seong-Rip, Lee Chi-Hoon, Kang Hyeong-Cheol, Song Young-Bo, Kim Hyung-Bae, Lee Young-Don
Fisheries Resources Research Institute, Jeju Special Self-Governing Province, Jeju 697-914, Republic of Korea.
Marine Science Institute, Jeju National University, Jeju 690-968, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):345-51. doi: 10.12717/DR.2013.17.4.345.
This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: 99.5±0.8%, DMSO 7.5%: 99.5±0.7%, and DMSO 10.0%: 99.6±0.6%). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: 96.2±2.3%, DMSO 7.5%: 95.3±3.6%, 10.0%: 96.6±1.8%) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm (98.4%±0.5) and DMSO concentration level of 5.0% (97.8±0.1%) were not significantly different. However, with 7.5% (97.2±0.6%) and 10.0% DMSO concentrations (95.9±0.2%) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; 66.8±1.8%, HR: 82.0±12.9%) and 2 years (FR; 78.5±14.8%, HR: 79.3±0.6%) cryopreserved sperm were lower than control (FR; 100%, HR: 91.1±3.6%) and 2 days cryopreserved sperm (FR; 99.6±0.6%, HR: 96.6±1.8%). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.
本研究根据冷冻保护剂（二甲基亚砜，DMSO）浓度和斜带石斑鱼（Epinephelus bruneus）精子的冷冻保存时间，研究了使用冷冻解冻精子进行人工授精后对受精率（FR）、孵化率（HR）和正常个体率的影响。使用冷冻解冻精子进行人工授精，分别在5.0%、7.5%、10.0%不同DMSO浓度下冷冻精子后，受精率分别为（DMSO 5.0%：99.5±0.8%，DMSO 7.5%：99.5±0.7%，DMSO 10.0%：99.6±0.6%）。结果与对照新鲜精子（100%）无显著差异。孵化率也无显著差异（DMSO 5.0%：96.2±2.3%，DMSO 7.5%：95.3±3.6%，10.0%：96.6±1.8%）。使用对照新鲜精子孵化后的正常个体率（98.4%±0.5）与DMSO浓度为5.0%时（97.8±0.1%）无显著差异。然而，DMSO浓度为7.5%（97.2±0.6%）和10.0%（95.9±0.2%）时，低于对照和DMSO 5.0%时观察到的孵化后正常个体率。在不同冷冻时间（2天、2年和3年）使用冷冻解冻精子进行人工授精，3年冷冻保存精子的10%DMSO受精率和孵化率（受精率；66.8±1.8%，孵化率：82.0±12.9%）以及2年冷冻保存精子的（受精率；78.5±14.8%，孵化率：79.3±0.6%）低于对照（受精率；100%，孵化率：91.1±3.6%）和2天冷冻保存精子（受精率；99.6±0.6%，孵化率：96.6±1.8%）。这些结果表明，斜带石斑鱼精子冷冻保存的适宜DMSO浓度范围为5%至10%，冷冻保存2至3年，冷冻精子可用于种苗生产。
