Aksoy Y, Yapanoğlu T, Aksoy H, Demircan B, Oztaşan N, Canakçi E, Malkoç I
Department of Urology, School of Medicine, Ataturk University, Erzurum, Turkey.
Arch Androl. 2006 Jul-Aug;52(4):319-23. doi: 10.1080/01485010500503587.
Male rats were equally divided into trained rest (TR), trained exhaustive exercise (TE), untrained rest (UR), and untrained exhaustive exercise (UE). Endurance training consisted of treadmill running for 1.5 h/d, 5 days a week for 8 weeks reaching the speed of 2.1 km/h at the fortieth week. For acute exhaustive exercise, graded treadmill running was conducted reaching the speed of 2.1 km/h at 95th min, 10% uphill, continued until exhaustion. Testicular tissue malondialdehyde (MDA), antioxidant potential (AOP) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR) and catalase (CAT) activities were determined. There was a slight decrease, but not significant, in the SOD activity in UE group compared to TE and TR groups. Activity of GSH-Px decreased in the UE group compared to UR, TR and TE groups. Acute exhaustive exercise did not affect testicular tissue GSH-Px activity in trained rats. Testicular tissue GST activity of the UE group was similar to TE group, but lower than UR and TR groups. In UE group, testicular tissue AOP values were lower than UR, TR and TE groups. The oxidative effects of acute exhaustive exercise on the rat testis decreased with endurance training. Endurance training prevents oxidative injuries by eliminating oxygen radicals and inhibiting lipid peroxidation via preventing decreases in antioxidant enzyme activities.
雄性大鼠被平均分为训练休息组（TR）、训练性力竭运动组（TE）、非训练休息组（UR）和非训练性力竭运动组（UE）。耐力训练包括每周5天、每天1.5小时的跑步机跑步，持续8周，在第40周时达到2.1公里/小时的速度。对于急性力竭运动，进行分级跑步机跑步，在第95分钟时达到2.1公里/小时的速度，坡度为10%上坡，持续至力竭。测定睾丸组织丙二醛（MDA）、抗氧化潜力（AOP）水平、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽-S-转移酶（GST）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）的活性。与TE组和TR组相比，UE组的SOD活性略有下降，但不显著。与UR组、TR组和TE组相比，UE组的GSH-Px活性降低。急性力竭运动对训练有素的大鼠睾丸组织GSH-Px活性没有影响。UE组的睾丸组织GST活性与TE组相似，但低于UR组和TR组。在UE组中，睾丸组织AOP值低于UR组、TR组和TE组。急性力竭运动对大鼠睾丸的氧化作用随着耐力训练而降低。耐力训练通过消除氧自由基和抑制脂质过氧化来预防氧化损伤，这是通过防止抗氧化酶活性下降来实现的。
