Taysi S, Oztasan N, Efe H, Polat M F, Gumustekin K, Siktar E, Canakci E, Akcay F, Dane S, Gul M
Department of Biochemistry and Clinical Biochemistry, University of Gaziantep, Faculty of Medicine, Gaziantep, Turkey.
Acta Physiol Hung. 2008 Dec;95(4):337-47. doi: 10.1556/APhysiol.95.2008.4.2.
The aim of this study was to investigate whether an 8-week treadmill training attenuates exercise-induced oxidative stress in rat liver. Male rats were divided into untrained and trained groups. Endurance training consisted of treadmill running at a speed of 2.1 km/h, 1.5 h/day, 5 days a week for 8 weeks. To see the effects of endurance training on acute exhaustive exercise induced oxidative stress, untrained and trained rats were further devided into two groups: animals killed at rest and those killed after acute exhaustive exercise, in which the rats run at 2.1 km/h (10% uphill) until exhaustion. Acute exhaustive exercise increased malondialdehyde level in untrained but not in trained rats. It decreased the activity of glutathione peroxidase and total (enzymatic plus non-enzymatic) superoxide scavenger activity in untrained rats and catalase activity in trained rats. However, it did not affect glutathione S-transferase, glutathione reductase, superoxide dismutase and non-enzymatic superoxide radical scavenger activities in both trained and untrained rats. On the other hand, endurance training decreased glutathione peroxidase and glutathione S-transferase activities. The results suggested that endurance training attenuated exercise-induced oxidative stress in liver, probably by preventing the decreases in glutathione peroxidase and total superoxide scavenger activities during exercise.
本研究的目的是调查为期8周的跑步机训练是否能减轻运动诱导的大鼠肝脏氧化应激。雄性大鼠被分为未训练组和训练组。耐力训练包括以2.1公里/小时的速度在跑步机上跑步，每天1.5小时，每周5天，持续8周。为了观察耐力训练对急性力竭运动诱导的氧化应激的影响，未训练和训练的大鼠进一步分为两组：在休息时处死的动物和在急性力竭运动后处死的动物，其中大鼠以2.1公里/小时（10%上坡）跑步直至力竭。急性力竭运动增加了未训练大鼠丙二醛水平，但训练大鼠未增加。它降低了未训练大鼠谷胱甘肽过氧化物酶活性和总（酶促加非酶促）超氧化物清除活性以及训练大鼠过氧化氢酶活性。然而，它对训练和未训练大鼠的谷胱甘肽S-转移酶、谷胱甘肽还原酶、超氧化物歧化酶和非酶促超氧化物自由基清除活性均无影响。另一方面，耐力训练降低了谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶活性。结果表明，耐力训练减轻了运动诱导的肝脏氧化应激，可能是通过防止运动期间谷胱甘肽过氧化物酶和总超氧化物清除活性的降低来实现的。
