腺苷对兔主动脉收缩及45Ca内流的抑制作用。

Inhibition of contractions and 45Ca influx by adenosine in rabbit aorta.

作者信息

Parija S C, Raviprakash V, Mishra S K

机构信息

Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar.

出版信息

J Pharm Pharmacol. 1991 Feb;43(2):134-7. doi: 10.1111/j.2042-7158.1991.tb06650.x.

DOI:10.1111/j.2042-7158.1991.tb06650.x

PMID:1672901

Abstract

The effects of adenosine on contractility and 45Ca-uptake in rabbit aorta have been examined and compared with those of nifedipine. Both adenosine (10(-5) M) and nifedipine (10(-8) M) inhibited contractions caused by CaCl2 (0.1-10 mM) in K(+)-depolarized preparations. Nifedipine (10(-7) M) significantly inhibited the contractions of noradrenaline (NA) at all doses (10(-8)-10(-4) M), while adenosine inhibited the contractions induced only by lower doses of NA (10(-8) and 10(-7) M) without affecting those at higher doses (greater than 10(-7) M). Adenosine (10(-8) M) and nifedipine (10(-7) M) inhibited K(+)-stimulated 45Ca-uptake by aortic strips by 75 and 100%, respectively. Similarity, NA (10(-4) M)-stimulated 45Ca influx was inhibited by about 70% by both these agents. The results suggest that adenosine inhibits inward Ca2+ movement involving both voltage-operated and receptor-operated calcium channels in rabbit aortic smooth muscle.

摘要

研究了腺苷对兔主动脉收缩性和45Ca摄取的影响，并与硝苯地平的作用进行了比较。腺苷（10(-5) M）和硝苯地平（10(-8) M）均抑制K(+)去极化制剂中由CaCl2（0.1 - 10 mM）引起的收缩。硝苯地平（10(-7) M）在所有剂量（10(-8) - 10(-4) M）下均显著抑制去甲肾上腺素（NA）的收缩，而腺苷仅抑制低剂量NA（10(-8)和10(-7) M）诱导的收缩，对高剂量（大于10(-7) M）的收缩无影响。腺苷（10(-8) M）和硝苯地平（10(-7) M）分别使主动脉条带K(+)刺激的45Ca摄取抑制75%和100%。同样，这两种药物均使NA（10(-4) M）刺激的45Ca内流抑制约70%。结果表明，腺苷抑制兔主动脉平滑肌中涉及电压门控和受体门控钙通道的内向Ca2+移动。