Naef Felix
Swiss Institute of Experimental Cancer Research (ISREC) and Swiss Institute of Bioinformatics (SIB), Epalinges, Switzerland.
Mol Syst Biol. 2005;1:2005.0019. doi: 10.1038/msb4100027. Epub 2005 Sep 13.
Recent findings about the core of the circadian oscillator in cyanobacteria are challenging the dogma that such clocks are driven through transcriptional-translational feedback regulation. Instead, the master pacemaker is independent of both transcription and translation, and consists of self-sustained oscillations in the phosphorylation status of the KaiC protein in vivo. Using a minimal cocktail of three recombinant proteins with adenosine triphosphate, the core clock was even reproduced in vitro. The so-born chemical oscillator could reproduce accurately temperature compensation and altered period phenotypes in mutants. This system now provides an ideal playground for rebuilding the circadian clock by adding successive components while understanding every single step with chemical resolution.
最近关于蓝藻生物钟振荡器核心的研究发现正在挑战这样一种教条，即此类生物钟是通过转录-翻译反馈调节驱动的。相反，主生物钟起搏器独立于转录和翻译，并且在体内由KaiC蛋白磷酸化状态的自持振荡组成。使用三种重组蛋白与三磷酸腺苷的最小混合物，甚至在体外重现了核心生物钟。由此产生的化学振荡器能够准确重现温度补偿以及突变体中改变的周期表型。这个系统现在为通过添加连续的组件来重建生物钟提供了一个理想的平台，同时以化学分辨率理解每一个步骤。
