牛磺酸转运体在成骨细胞中表达。

Taurine transporter is expressed in osteoblasts.

作者信息

Yuan L-Q, Xie H, Luo X-H, Wu X-P, Zhou H-D, Lu Y, Liao E-Y

机构信息

Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China.

出版信息

Amino Acids. 2006 Sep;31(2):157-63. doi: 10.1007/s00726-005-0313-7. Epub 2006 May 26.

DOI:10.1007/s00726-005-0313-7

PMID:16729199

Abstract

Taurine influences bone metabolism and is taken up by cells via a specific transport system, the taurine transporter (TAUT). We report a link between taurine and bone homeostasis by demonstrating transcription and translation of TAUT in bone-forming cells. TAUT was expressed in human primary osteoblasts, the human osteosarcoma osteoblast-like cell line MG63, and the mouse osteoblastic cell line MC3T3-E1. Immunostaining with polyclonal antibodies also demonstrated the presence of TAUT in both human and murine osteoblasts. TAUT mRNA expression and [(3)H]taurine uptake increased during differentiation of MG63 cells in culture. Supplementation of culture medium with taurine enhanced alkaline phosphatase activity and osteocalcin secretion. The regulation and detailed function of taurine and TAUT in bone remain unclear, but our findings suggest a functional role for them in bone homeostasis.

摘要

牛磺酸影响骨代谢，并通过特定的转运系统——牛磺酸转运体（TAUT）被细胞摄取。我们通过证明TAUT在成骨细胞中的转录和翻译，报道了牛磺酸与骨稳态之间的联系。TAUT在人原代成骨细胞、人骨肉瘤成骨样细胞系MG63和小鼠成骨细胞系MC3T3-E1中表达。用多克隆抗体进行免疫染色也证明了TAUT在人和小鼠成骨细胞中的存在。在培养的MG63细胞分化过程中，TAUT mRNA表达和[³H]牛磺酸摄取增加。在培养基中添加牛磺酸可增强碱性磷酸酶活性和骨钙素分泌。牛磺酸和TAUT在骨中的调节和详细功能尚不清楚，但我们的研究结果表明它们在骨稳态中具有功能性作用。