Adam Maja, Contzen Matthias, Horlacher Sabine, Rau Jörg
Landesgesundheitsamt Stuttgart, Regierungspräsidium.
Berl Munch Tierarztl Wochenschr. 2006 May-Jun;119(5-6):209-15.
The identification of thermotolerant campylobacters in official food control in the state of Baden-Wuerttemberg has been traditionally performed using the cultural procedure as described in the ISO-Norm 10272:1995. Analysis thus took 5-6 days to complete. Additionally diagnostic problems caused by the accompanying flora as well as the resistance to nalidixic acid occured. Within the scope of this study these problems could be solved by introducing a filtration step for the reduction of the accompanying flora and by performing the indoxyl acetate-hydrolysis-test in addition to the antibiotic-resistance-test. Besides various PCR protocols for the identification of thermotolerant campylobacters from food were established as an alternative to the cultural procedure, providing reliable results within two days. Furthermore, infrared spectroscopy was tested for the identification of Campylobacter isolates. Using this technique and with the help of a suitable data base, bacterial pure cultures can be differentiated within 2 hours. Among others 356 samples of raw poultry meat were tested with the newly established procedures as well as with the classical cultural method, showing that 32% of the samples were Campylobacter spp. positive. 37% of these isolates were resistant against nalidixic acid. This indicates that the development of resistances in Campylobacter spp. in Germany follows the same trend described for other European countries.
在巴登-符腾堡州的官方食品检测中，传统上一直采用ISO标准10272:1995中描述的培养方法来鉴定耐热弯曲杆菌。因此，分析需要5至6天才能完成。此外，还出现了由伴随菌群引起的诊断问题以及对萘啶酸的耐药性问题。在本研究范围内，这些问题可以通过引入一个过滤步骤以减少伴随菌群，并通过在抗生素耐药性测试之外进行醋酸吲哚酚水解试验来解决。此外，还建立了各种用于从食品中鉴定耐热弯曲杆菌的PCR方法，作为培养方法的替代方法，可在两天内提供可靠结果。此外，还对红外光谱法用于弯曲杆菌分离株的鉴定进行了测试。使用该技术并借助合适的数据库，可在两小时内区分细菌纯培养物。其中，356份生禽肉样品用新建立的方法以及经典培养方法进行了检测，结果显示32%的样品弯曲杆菌属呈阳性。这些分离株中有37%对萘啶酸耐药。这表明德国弯曲杆菌属耐药性的发展与其他欧洲国家描述的趋势相同。
