Praakle-Amin Kristi, Roasto Mati, Korkeala Hannu, Hänninen Marja-Liisa
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014 University of Helsinki, Finland.
Int J Food Microbiol. 2007 Feb 28;114(1):105-12. doi: 10.1016/j.ijfoodmicro.2006.10.034. Epub 2006 Dec 19.
In the present study, the Campylobacter isolates from retail poultry meat in Estonia were sero- and genotyped, and the antimicrobial susceptibility was determined. Forty-eight chicken (36 Estonian, 12 imported) and 22 turkey (imported) Campylobacter isolates from 580 raw broiler chicken (396 Estonian, 184 imported) and 30 turkey (imported) meat samples were studied. Of the isolates, 64 were C. jejuni, 4 C. coli, and 2 Campylobacter spp. Penner serotyping of 54 C. jejuni isolates revealed 11 different serotypes, and 22% of the isolates were nontypeable by the commercial antisera. The most common serotypes O:1,44; O:21, and O:55 accounted for 28%, 13%, and 13% of the isolates, respectively. Differences in serotype distribution were seen for chicken and turkey isolates. Genotypic characterization of all Campylobacter isolates (n=70) was performed by pulsed-field gel electrophoresis (PFGE). SmaI and KpnI yielded 29 and 34 PFGE types, respectively, revealing high diversity among isolates. The serotype distribution did not show an association with the origin of the sample, but the majority of the isolates sharing a similar PFGE genotype originated from one country. High levels of resistance to ciprofloxacin (66%), nalidixic acid (66%), tetracycline (44%), ampicillin (34%), and erythromycin (14%) were detected among the 70 Campylobacter isolates. The simultaneous resistance to two or three antimicrobial agents occurred in 60% of the isolates. The Campylobacter isolates from turkey meat had higher resistance to ampicillin, ciprofloxacin, nalidixic acid, and tetracycline than those from chicken meat. None of the chicken isolates were resistant to gentamicin, and no turkey isolates to erythromycin or gentamicin.
在本研究中，对爱沙尼亚零售禽肉中的弯曲杆菌分离株进行了血清分型和基因分型，并测定了其抗菌药敏性。研究了从580份生肉鸡（396份爱沙尼亚产，184份进口）和30份火鸡（进口）肉样中分离出的48株鸡（36株爱沙尼亚产，12株进口）和22株火鸡（进口）弯曲杆菌分离株。在这些分离株中，64株为空肠弯曲菌，4株为结肠弯曲菌，2株为弯曲杆菌属。对54株空肠弯曲菌分离株进行彭纳血清分型，发现了11种不同血清型，22%的分离株无法用商业抗血清分型。最常见的血清型O:1,44、O:21和O:55分别占分离株的28%、13%和13%。鸡肉和火鸡分离株的血清型分布存在差异。对所有弯曲杆菌分离株（n = 70）进行了脉冲场凝胶电泳（PFGE）基因分型。SmaI和KpnI分别产生了29种和34种PFGE型，表明分离株间具有高度多样性。血清型分布与样本来源无关联，但大多数具有相似PFGE基因型的分离株来自同一个国家。在70株弯曲杆菌分离株中检测到对环丙沙星（66%）、萘啶酸（66%）、四环素（44%）、氨苄西林（34%）和红霉素（14%）的高耐药水平。60%的分离株同时对两种或三种抗菌药物耐药。火鸡肉中的弯曲杆菌分离株对氨苄西林、环丙沙星、萘啶酸和四环素的耐药性高于鸡肉中的分离株。鸡肉分离株均对庆大霉素不耐药，火鸡分离株对红霉素或庆大霉素均不耐药。
