Huang Cuihong, Wang Xiaoying, Wang Rongjiang, Xue Kun, Yan Fengming, Xu Chongren
College of Life Sciences, Peking University, Beijing, P R China.
Z Naturforsch C J Biosci. 2006 Mar-Apr;61(3-4):257-62. doi: 10.1515/znc-2006-3-417.
Contents of three 1,4-benzoxazin-3-ones in tissue samples from different parts (young leaf, second leaf, old leaf, stem and root) of young maize plants of 4-leaves stage, fed by the third instar larvae of the Asian corn borer, Ostrinia furnacalis (Guenée), were analyzed by high-performance liquid chromatography-mass spectroscopy (HPLC-MS). Samples were taken immediately (set A) or 48 h (set B) after larvae had fed on the second leaf for 48 h. The three 1,4-benzoxazin-3-ones investigated in our experiments were 2,4-dihydroxy-7-methoxy-1,4(2H)-benzoxazin-3-one (DIMBOA), 2,4-dihydroxy-1,4(2H)-benzoxazin-3-one (DIBOA) and 2-hydroxy-7-methoxy-1,4(2H)-benzoxazin-3-one (HMBOA). In samples of set A, the levels of DIMBOA and HMBOA were significantly lifted in the old leaf (L3) and young leaf (L1), respectively, while amounts of these two chemicals in other plant parts were not significantly different between larvae-fed plants and intact plants. Concentrations of DIBOA in each plant part remained unchanged. In samples of set B, no concentration differences for any of these three 1,4-benzoxazin-3-ones between larvae-fed plants and controls were observed in any plant part. The feeding of the Asian corn borer seems to have limited effects on induction of these three 1,4-benzoxazin-3-ones in young maize plants of the variety investigated.
采用高效液相色谱 - 质谱联用仪（HPLC - MS）分析了4叶期玉米幼苗不同部位（幼叶、第二叶、老叶、茎和根）的组织样本中3种1,4 - 苯并恶嗪 - 3 - 酮的含量。这些样本是在亚洲玉米螟（Ostrinia furnacalis (Guenée)）三龄幼虫取食第二叶48小时后立即采集的（A组），或者在取食48小时后48小时采集的（B组）。在我们的实验中研究的3种1,4 - 苯并恶嗪 - 3 - 酮分别是2,4 - 二羟基 - 7 - 甲氧基 - 1,4(2H) - 苯并恶嗪 - 3 - 酮（DIMBOA）、2,4 - 二羟基 - 1,4(2H) - 苯并恶嗪 - 3 - 酮（DIBOA）和2 - 羟基 - 7 - 甲氧基 - 1,4(2H) - 苯并恶嗪 - 3 - 酮（HMBOA）。在A组样本中，DIMBOA和HMBOA的含量分别在老叶（L3）和幼叶（L1）中显著升高，而在其他植物部位，这两种化学物质在受幼虫取食的植物和完整植物之间没有显著差异。各植物部位中DIBOA的浓度保持不变。在B组样本中，在任何植物部位均未观察到受幼虫取食的植物与对照之间这3种1,4 - 苯并恶嗪 - 3 - 酮中的任何一种存在浓度差异。亚洲玉米螟的取食似乎对所研究品种的玉米幼苗中这3种1,4 - 苯并恶嗪 - 3 - 酮的诱导作用影响有限。
