用气相色谱法定量分析玉米中的 1,4-苯并恶嗪-3-酮。
Quantitation of 1,4-Benzoxazin-3-ones in Maize by Gas-Liquid Chromatography.
机构信息
Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin, 53706.
出版信息
Plant Physiol. 1979 Jan;63(1):14-9. doi: 10.1104/pp.63.1.14.
A gas-liquid chromatographic (GLC) procedure is reported for the quantitation of the trimethylsilyl (TMS) derivatives of substituted 2-hydroxy-2H-1,4-benzoxazin-3(4H)-ones (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one[HBOA]; 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one[HMBOA];2,4- dihydroxy-2H-1,4-benzoxazin-3(4H)-one[DIBOA]; 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one[DIMBOA]; and 2,4-dihydroxy-7,8-dimethoxy-2H-1,4-benzoxazin-3(4H)-one[DIM (2)BOA]) found in maize (Zea mays L.) extracts. Derivatized samples were chromatographed on columns with liquid phases of 2% DC-11 and 3% OV-17 and detected by flame ionization. Internal standards were methyl palmitate and methyl stearate on DC-11 and methyl behenate on OV-17. Detector response was linear to at least 5 nanomoles for TMS(2)-HBOA and TMS(2)-DIBOA and to 19 nanomoles for TMS(2)-DIMBOA. Standard errors of 2% or less were obtained when four replicate samples were analyzed. For each of the 15 maize lines examined, the amount of DIMBOA determined by GLC was directly proportional to the amount of ferric chloride-reactive material determined colorimetrically.
一种气-液色谱（GLC）程序被报道用于定量取代的 2-羟基-2H-1,4-苯并恶嗪-3(4H)-酮（2-羟基-2H-1,4-苯并恶嗪-3(4H)-酮[HBOA]；2-羟基-7-甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮[HMBOA]；2,4-二羟基-2H-1,4-苯并恶嗪-3(4H)-酮[DIBOA]；2,4-二羟基-7-甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮[DIMBOA]；和 2,4-二羟基-7,8-二甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮[DIM(2)BOA]）在玉米（Zea mays L.）提取物中发现。衍生化样品在具有 2% DC-11 和 3% OV-17 的液相的柱子上进行色谱分离，并通过火焰电离检测。内标物在 DC-11 上为肉豆蔻酸甲酯和硬脂酸甲酯，在 OV-17 上为山嵛酸甲酯。对于 TMS(2)-HBOA 和 TMS(2)-DIBOA，至少到 5 毫摩尔的检测器响应是线性的，对于 TMS(2)-DIMBOA，到 19 毫摩尔的检测器响应是线性的。当分析四个重复样品时，标准误差为 2%或更小。对于检查的 15 个玉米系，通过 GLC 确定的 DIMBOA 量与通过比色法确定的三氯化铁反应性物质的量成正比。
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