一种用于微小隐孢子虫卵囊免疫标记的改进电子显微镜技术。
An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts.
作者信息
Jenkins Mark C, Murphy Charles, Trout James, Fayer Ronald
机构信息
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA.
出版信息
J Parasitol. 2006 Apr;92(2):403-5. doi: 10.1645/GE-3539RN.1.
A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa.
开发了一种用于免疫标记微小隐孢子虫卵囊的技术，以便随后通过透射电子显微镜进行观察。开发此方法是为了保持卵囊壁的结构完整性并改善内部成分的固定。改进后的固定和包埋方法允许对未脱囊和已脱囊的微小隐孢子虫卵囊进行高效免疫标记，并且可能适用于其他形成卵囊和包囊的原生动物。
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