一种用于预防猪细小病毒引起的繁殖障碍的新型重组病毒样颗粒疫苗。
A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure.
作者信息
Antonis Adriaan F G, Bruschke Christianne J M, Rueda Paloma, Maranga Luis, Casal J Ignacio, Vela Carmen, Hilgers Luuk A Th, Belt Peter B G M, Weerdmeester Klaas, Carrondo Manuel J T, Langeveld Jan P M
机构信息
Animal Sciences Group (ASG, Previously ID-Lelystad), P.O. Box 65, 8200 AB Lelystad, The Netherlands.
出版信息
Vaccine. 2006 Jun 29;24(26):5481-90. doi: 10.1016/j.vaccine.2006.03.089. Epub 2006 Apr 18.
A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7microg of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines.
一种针对猪细小病毒（PPV）的新型疫苗，由杆状病毒表达载体系统（BEVS）在工业规模生产的重组病毒样颗粒（PPV-VLPs）组成，对其免疫原性和保护效力进行了测试。在水包油佐剂中含有亚微克量PPV-VLPs的制剂，在用作参考模型的豚鼠和目标物种猪中均引发了高血清抗体滴度。用0.7微克这种抗原进行单次免疫，在用该病毒的强毒株攻击后，可对PPV感染提供完全的胎儿保护。此外，即使在存在温和佐剂的情况下，这些PPV-VLPs的保护作用也非常出色。这种重组亚单位疫苗克服了传统PPV疫苗的一些缺点。
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