Le Doaré K, Akerman S, Holland P R, Lasalandra M P, Bergerot A, Classey J D, Knight Y E, Goadsby P J
Headache Group, Institute of Neurology, The National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK.
Neurosci Lett. 2006 Jul 31;403(1-2):73-7. doi: 10.1016/j.neulet.2006.04.049. Epub 2006 May 24.
Stimulation of the greater occipital nerve produces excitation of second order neurons in the trigeminocervical complex. Given that neck pain is very common in primary headache disorders, this convergent excitation may play a role in pain referral from cervical structures. While previous studies have demonstrated a physiological model for this convergence, this study sought an anatomical approach to examine the distribution of second order neurons in the trigeminocervical complex receiving greater occipital nerve input. In addition, the role of glutamatergic NMDA receptor activation within the trigeminocervical complex in response to cervical afferents was studied. Noxious stimulation of the occipital muscle in rat using mustard oil and mineral oil produced significantly altered Fos expression in the trigeminocervical complex compared with the surgical control (H(4)=31.3, P<0.001, Kruskal-Wallis). Baseline expression was 11 (median, range 4, 17) fos positive cells in the trigeminocervical complex, occipital muscle treated with mustard oil produced 23 (17, 33) and mineral oil a smaller effect of 19 (15, 25) fos positive cells, respectively (P=0.046). The effects of both mustard and mineral oil were reversed by the NMDA-receptor antagonist MK801. This study introduces a model for examining trigeminocervical complex activity after occipital afferent stimulation in the rat that has good anatomical resolution and demonstrates involvement of glutamatergic NMDA receptors at this important synapse.
刺激枕大神经会激发三叉颈复合体中的二级神经元。鉴于颈部疼痛在原发性头痛疾病中非常常见，这种汇聚性兴奋可能在颈部结构的疼痛牵涉中起作用。虽然先前的研究已经证明了这种汇聚的生理模型，但本研究寻求一种解剖学方法来检查三叉颈复合体中接受枕大神经输入的二级神经元的分布。此外，还研究了三叉颈复合体中谷氨酸能NMDA受体激活对颈部传入神经的反应作用。与手术对照组相比，用芥子油和矿物油对大鼠枕部肌肉进行有害刺激后，三叉颈复合体中的Fos表达发生了显著变化（H(4)=31.3，P<0.001，Kruskal-Wallis检验）。三叉颈复合体中基线表达为11（中位数，范围4，17）个Fos阳性细胞，芥子油处理的枕部肌肉产生23（17，33）个，矿物油产生较小影响，为19（15，25）个Fos阳性细胞（P=0.046）。NMDA受体拮抗剂MK801可逆转芥子油和矿物油的作用。本研究引入了一种用于检查大鼠枕部传入神经刺激后三叉颈复合体活动的模型，该模型具有良好的解剖分辨率，并证明了谷氨酸能NMDA受体在这一重要突触中的参与。
