Nimesh Surendra, Kumar Rupesh, Chandra Ramesh
Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Int J Pharm. 2006 Aug 31;320(1-2):143-9. doi: 10.1016/j.ijpharm.2006.03.050. Epub 2006 May 5.
Polyallylamine (PAA), a less investigated polycationic polymer along with polyanionic dextran sulfate (DS) has been complexed with DNA into self assembling PAA-DS-DNA complexes with zinc as stabilizing agent. The complexes prepared were characterized by dynamic light scattering and atomic force microscopy and found to have average hydrodynamic diameter of 150nm with polydispersity below 0.2, i.e. nanoplexes with narrow size distribution. As expected, the zeta potential values increased with the increase in the PAA in the nanoplexes. Further, PAA-DS-DNA nanoplexes were investigated for in vitro transfecting efficacy on HEK 293 cells. The nanoplexes were found to have better gene transfer efficacy compared to the DNA-PAA complex and commercially available transfecting agent lipofectin. The cytotoxicity was also decreased considerably as revealed by MTT colorimetric assay. The nanoplexes prepared with PAA to DS ratio of 2 were found to be most efficient transfecting agent.
聚烯丙胺（PAA）是一种研究较少的聚阳离子聚合物，它与聚阴离子硫酸葡聚糖（DS）一起，以锌作为稳定剂，与DNA复合形成自组装的PAA-DS-DNA复合物。通过动态光散射和原子力显微镜对制备的复合物进行表征，发现其平均流体动力学直径为150nm，多分散性低于0.2，即具有窄尺寸分布的纳米复合物。正如预期的那样，纳米复合物中PAA的增加会使zeta电位值升高。此外，还研究了PAA-DS-DNA纳米复合物对HEK 293细胞的体外转染效率。结果发现，与DNA-PAA复合物和市售转染剂脂质体相比，该纳米复合物具有更好的基因转移效率。MTT比色法显示，其细胞毒性也显著降低。发现PAA与DS比例为2制备的纳米复合物是最有效的转染剂。
