Du Junrong, Bai Bo, Kuang Xi, Yu Yan, Wang Chenyuen, Ke Ya, Xu Youjia, Tzang Alexander H C, Qian Zhong Ming
Department of Applied Biology and Chemical Technology, Center of Scientific Development of TCM-based Products, The Hong Kong Polytechnic University, Hong Kong, PR China.
J Ethnopharmacol. 2006 Nov 3;108(1):54-8. doi: 10.1016/j.jep.2006.04.011. Epub 2006 Apr 28.
In the present study, the effects of ligustilide (LIG) on uterine contraction in vitro were investigated. In isolated rat uterine, LIG (2-8 microg/ml) inhibited the spontaneous periodic contraction in a concentration-dependent manner (EC(50)=4.4 microg/ml, 95% confidence interval 2.7-6.1 microg/ml), and attenuated prostaglandin F2alpha (PGF(2)alpha)- or acetylcholine chloride (Ach)-induced uterine contractions. At 8 microg/ml, LIG nearly completely blocked the PGF(2)alpha-induced contractions (95.3%). In the case of Ach-induced contraction, about 73.9% was inhibited by LIG at this dosage. It was also observed that LIG affected significantly oxytocin-induced increase in the contraction of uterine horns that were incubated not only in the Locke solution but also in a Ca(2+)-free solution. In addition, LIG caused concentration-dependent inhibition of uterine contraction induced by K(+) (56.3 Mm) depolarization, reaching the significant level at 2 microg/ml (EC(50)=3.3 microg/ml, 95% confidence interval 2.5-4.1 microg/ml). The findings clearly show that LIG has multiple effects on the uterine smooth muscles, suggesting that LIG possesses a non-specific antispasmodic function. The data also imply strongly that LIG is one of active ingredients of Danggui and has the potential to be developed into an effective drug for the prevention and treatment of primary dysmenorrhoea.
在本研究中，考察了藁本内酯（LIG）对体外子宫收缩的影响。在离体大鼠子宫中，LIG（2 - 8微克/毫升）以浓度依赖性方式抑制自发性周期性收缩（半数有效浓度[EC(50)] = 4.4微克/毫升，95%置信区间为2.7 - 6.1微克/毫升），并减弱前列腺素F2α（PGF(2)α）或氯化乙酰胆碱（Ach）诱导的子宫收缩。在8微克/毫升时，LIG几乎完全阻断PGF(2)α诱导的收缩（95.3%）。对于Ach诱导的收缩，在此剂量下LIG抑制约73.9%。还观察到，LIG显著影响催产素诱导的子宫角收缩增加，子宫角不仅在洛克溶液中孵育，也在无钙溶液中孵育。此外，LIG对K⁺（56.3毫摩）去极化诱导的子宫收缩产生浓度依赖性抑制，在2微克/毫升时达到显著水平（EC(50)=3.3微克/毫升，95%置信区间为2.5 - 4.1微克/毫升）。这些发现清楚地表明，LIG对子宫平滑肌有多种作用，提示LIG具有非特异性解痉功能。数据还强烈暗示，LIG是当归的活性成分之一，有潜力开发成为预防和治疗原发性痛经的有效药物。
