Lukens Michaël V, Claassen Erwin A W, de Graaff Patricia M A, van Dijk Mariska E A, Hoogerhout Peter, Toebes Mireille, Schumacher Ton N, van der Most Robbert G, Kimpen Jan L L, van Bleek Grada M
Department of Pediatrics, The Wilhelmina Children's Hospital, University Medical Center, KE.04.133.1, Lundlaan 6, 3584 EA Utrecht, The Netherlands.
Virology. 2006 Aug 15;352(1):157-68. doi: 10.1016/j.virol.2006.04.023. Epub 2006 May 30.
The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4+ and CD8+ T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8+ T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8+ T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.
人类呼吸道合胞病毒感染的BALB/c小鼠模型对我们理解CD4+和CD8+ T细胞在免疫保护和致病性免疫反应中的相对作用做出了重大贡献。为了能够比较不同小鼠品系中针对呼吸道合胞病毒（RSV）的T细胞反应，并通过使用大多基于C57BL/6背景的转基因和基因敲除小鼠来剖析免疫机制，我们对C57BL/6小鼠肺实质、气道和脾脏在初次和二次反应期间CD8+ T细胞的特异性、水平和功能能力进行了表征。在初次反应期间，可识别出源自基质、融合、核衣壳和附着蛋白的表位，而二次反应主要集中在基质表位上。C57BL/6小鼠对人RSV感染的易感性低于BALB/c小鼠，但我们发现其肺和支气管肺泡灌洗中的CD8+ T细胞反应与BALB/c小鼠中描述的反应相当。
