Gadekar S, Nemati M, Hill G A
Department of Chemical Engineering, College of Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Sask., Canada S7N 5A9.
Water Res. 2006 Jul;40(12):2436-46. doi: 10.1016/j.watres.2006.04.007. Epub 2006 May 30.
Aqueous phase biooxidation of sulphide by the novel sulphide-oxidizing bacterium Thiomicrospira sp. CVO was studied in batch and continuous systems. CVO was able to oxidize sulphide at concentrations as high as 19 mM. Sulphide biooxidation occurred in two distinct phases, one resulting in the formation of sulphur and possibly other dissolved sulphur compounds rather than sulphate, followed by sulphate formation. The specific growth rate of CVO in the first and second phases were 0.17-0.27 and 0.04-0.05 h(-1), respectively. Nitrite accumulated in the culture during the first phase and was consumed during the second phase. The composition of end-products was influenced by the ratio of sulphide to nitrate initial concentrations. At a ratio of 0.28, sulphate represented 93% of the reaction products, while with a ratio of 1.6 the conversion of sulphide to sulphate was only 9.3%. In the continuous bioreactor, complete removal of sulphide was observed at sulphide volumetric loading rates as high as 1.6mM/h (residence time of 10h). Overall sulphide removal efficiency decreased continuously upon further increases in volumetric loading rate. However, the volumetric removal rate increased until a maximum value of 2.4mM/h was obtained at a loading rate of 3.2mM/h. The corresponding sulphide conversion and residence time were 76% and 5.6h, respectively. As expected from the high ratio of sulphide to nitrate loading rates (1.7-1.9 mM/h), no sulphate was formed in the continuous reactor. Using the experimental data the value of maximum specific growth rate, saturation constant, decay coefficient, maintenance coefficient and yield were determined to be 0.36 h(-1), 1.99 mM sulphide, 0.0014 h(-1), 0.078 mmol sulphide/mg ATPh and 0.018 mg ATP/mmol sulphide, respectively.
在分批和连续系统中研究了新型硫化物氧化细菌嗜硫微螺菌属CVO对硫化物的水相生物氧化作用。CVO能够氧化浓度高达19 mM的硫化物。硫化物生物氧化分两个不同阶段进行，第一阶段导致硫以及可能的其他溶解态硫化合物而非硫酸盐的形成，随后是硫酸盐的形成。CVO在第一阶段和第二阶段的比生长速率分别为0.17 - 0.27 h⁻¹和0.04 - 0.05 h⁻¹。亚硝酸盐在第一阶段在培养物中积累，并在第二阶段被消耗。终产物的组成受硫化物与硝酸盐初始浓度之比的影响。当比例为0.28时，硫酸盐占反应产物的93%，而当比例为1.6时，硫化物向硫酸盐的转化率仅为9.3%。在连续生物反应器中，当硫化物体积负荷率高达1.6 mM/h（停留时间为10 h）时，观察到硫化物被完全去除。随着体积负荷率进一步增加，总体硫化物去除效率持续下降。然而，体积去除率一直增加，直到在3.2 mM/h的负荷率下达到2.4 mM/h的最大值。相应的硫化物转化率和停留时间分别为76%和5.6 h。正如从高硫化物与硝酸盐负荷率之比（1.7 - 1.9 mM/h）所预期的那样，连续反应器中未形成硫酸盐。利用实验数据确定最大比生长速率、饱和常数、衰减系数、维持系数和产率的值分别为0.36 h⁻¹、1.99 mM硫化物、0.0014 h⁻¹、0.078 mmol硫化物/mg ATP h和0.018 mg ATP/mmol硫化物。
