Department of Chemical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Canada.
Bioresour Technol. 2010 Nov;101(21):8109-18. doi: 10.1016/j.biortech.2010.06.037. Epub 2010 Jul 2.
Microbial cultures originated from an oil reservoir were used in three biofilm reactors and effects of sulphide and nitrate loading rates and molar loading ratio on the removal of sulphide, nitrate and acetate, and composition of end products were investigated. Application of biofilms improved sulphide and nitrate removal rates significantly when compared with freely suspended cells. Maximum sulphide and nitrate removal rates under autotrophic conditions were 30.0 and 24.4 mM h(-1), respectively (residence time: 0.5h). Oxidation of acetate occurred only at nitrate to sulphide molar loading ratios around 0.7 or higher when nitrate was present at levels higher than that required for oxidation of sulphide to sulphur. Conversion of sulphide to sulphate increased from 0% to 66% as nitrate to sulphide molar loading ratio was increased from 0.34 to 3.98. The highest nitrate and acetate removal rates in the bioreactor operated under heterotrophic conditions were 183.2 and 88.0 mM h(-1), respectively (residence time: 0.8h).
从油藏中分离得到的微生物菌群被用于三个生物膜反应器中，考察了硫化物和硝酸盐的加载速率以及摩尔加载比对于去除硫化物、硝酸盐和乙酸盐的效果，以及产物的组成。与自由悬浮细胞相比，生物膜的应用显著提高了硫化物和硝酸盐的去除速率。在自养条件下，最大的硫化物和硝酸盐去除速率分别为 30.0 和 24.4 mM h(-1)（停留时间：0.5h）。只有当硝酸盐的浓度高于将硫化物氧化为硫所需的浓度，并且硝酸盐与硫化物的摩尔比在 0.7 左右或更高时，才会发生乙酸盐的氧化。随着硝酸盐与硫化物的摩尔比从 0.34 增加到 3.98，硫化物转化为硫酸盐的比例从 0%增加到 66%。在异养条件下运行的生物反应器中，硝酸盐和乙酸盐的最高去除速率分别为 183.2 和 88.0 mM h(-1)（停留时间：0.8h）。
