Yu Christine K Y, Lam Corey N W, Springob Karin, Schmidt Jüergen, Chu Ivan K, Lo Clive
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
Plant Cell Physiol. 2006 Jul;47(7):1017-21. doi: 10.1093/pcp/pcj061. Epub 2006 May 26.
Sorghum SbSTS1 was the first example of a stilbene synthase gene in monocots. Previously, we demonstrated that the gene was involved in defense responses. To examine its biochemical function in planta, SbSTS1 was overexpressed in transgenic Arabidopsis. Metabolite analysis revealed that cis-resveratrol glucoside (piceid) accumulated as the major stilbene in the transgenic lines. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in selected reaction monitoring mode, up to 580 microg g(-1) FW of cis-piceid were detected in 2-week-old plants, which represent a convenient source of the cis-isomers for pharmacological investigations. Our results also suggested the presence of unknown stilbene isomerase activities in Arabidopsis.
高粱SbSTS1是单子叶植物中第一个芪合酶基因的实例。此前，我们证明该基因参与防御反应。为了研究其在植物中的生化功能，SbSTS1在转基因拟南芥中过表达。代谢物分析表明，顺式白藜芦醇葡萄糖苷（云杉新苷）作为主要芪类物质在转基因株系中积累。采用选择反应监测模式的液相色谱-串联质谱法（LC-MS/MS），在两周大的植株中检测到高达580 μg g(-1) FW的顺式云杉新苷，这为药理学研究提供了方便的顺式异构体来源。我们的结果还表明拟南芥中存在未知的芪异构酶活性。
