McNamee Cathy E, Pyo Nayoung, Higashitani Ko
Department of Chemical Engineering, Kyoto University-Katsura, Kyoto 615-8510, Japan.
Biophys J. 2006 Sep 1;91(5):1960-9. doi: 10.1529/biophysj.106.082420. Epub 2006 May 26.
We investigated the effect of the charge and the hydrophobicity of drug delivery system (DDS) carriers on their specificity to living malignant melanoma B16F10 cells with the atomic force microscope. To model various nanoparticle DDS carriers, we used silica particles that were modified with silane coupling agents. We then measured the compression and decompression forces between the modified colloid probes and the living B16F10 cell in a physiological buffer as a function of their separation distances. The maximum adhesive force on decompression was related to the strength of the specificity of the DDS to the malignant cell. A comparison of the average maximum adhesive force of each functionality group surprisingly showed that negatively charged surfaces and hydrophobic modified surfaces all had similar low values. Additionally, we saw the unexpected result that there was no observable dependence on the degree of hydrophobicity of the probe surface to a B16F10 cell. Only the positively charged particle gave a strong adhesive force with the B16F10 cell. This indicated that DDS carriers with positive charges appeared to have the highest affinity for malignant melanoma cells and that the use of hydrophobic materials unexpectedly did not improve their affinity.
我们使用原子力显微镜研究了药物递送系统（DDS）载体的电荷和疏水性对其与活体恶性黑色素瘤B16F10细胞特异性的影响。为了模拟各种纳米颗粒DDS载体，我们使用了经硅烷偶联剂改性的二氧化硅颗粒。然后，我们在生理缓冲液中测量了改性胶体探针与活体B16F10细胞之间的压缩力和减压力，作为它们分离距离的函数。减压时的最大粘附力与DDS对恶性细胞特异性的强度有关。对每个官能团的平均最大粘附力的比较令人惊讶地发现，带负电荷的表面和疏水改性表面的值都很低且相似。此外，我们得到了一个意外的结果，即未观察到探针表面疏水性程度对B16F10细胞有依赖性。只有带正电荷的颗粒与B16F10细胞产生了很强的粘附力。这表明带正电荷的DDS载体似乎对恶性黑色素瘤细胞具有最高的亲和力，而使用疏水性材料意外地并未提高它们的亲和力。
