Yallapu Murali M, Katti Kalpana S, Katti Dinesh R, Mishra Sanjay R, Khan Sheema, Jaggi Meena, Chauhan Subhash C
Department of Pharmaceutical Sciences and Center for Cancer Research, College of Pharmacy, University of Tennessee Health Science Center, Memphis, Tennessee, 38163.
Med Res Rev. 2015 Jan;35(1):198-223. doi: 10.1002/med.21329. Epub 2014 Aug 18.
The biomechanical properties of cells and tissues may be instrumental in increasing our understanding of cellular behavior and cellular manifestations of diseases such as cancer. Nanomechanical properties can offer clinical translation of therapies beyond what are currently employed. Nanomechanical properties, often measured by nanoindentation methods using atomic force microscopy, may identify morphological variations, cellular binding forces, and surface adhesion behaviors that efficiently differentiate normal cells and cancer cells. The aim of this review is to examine current research involving the general use of atomic force microscopy/nanoindentation in measuring cellular nanomechanics; various factors and instrumental conditions that influence the nanomechanical properties of cells; and implementation of nanoindentation methods to distinguish cancer cells from normal cells or tissues. Applying these fundamental nanomechanical properties to current discoveries in clinical treatment may result in greater efficiency in diagnosis, treatment, and prevention of cancer, which ultimately can change the lives of patients.
细胞和组织的生物力学特性可能有助于增进我们对细胞行为以及诸如癌症等疾病的细胞表现的理解。纳米力学特性可为目前所采用的治疗方法提供临床转化。纳米力学特性通常通过使用原子力显微镜的纳米压痕方法来测量，它可以识别形态变化、细胞结合力和表面粘附行为，从而有效地区分正常细胞和癌细胞。本综述的目的是研究当前涉及原子力显微镜/纳米压痕在测量细胞纳米力学方面的一般应用的研究；影响细胞纳米力学特性的各种因素和仪器条件；以及利用纳米压痕方法区分癌细胞与正常细胞或组织。将这些基本的纳米力学特性应用于当前临床治疗中的发现，可能会提高癌症诊断、治疗和预防的效率，最终改变患者的生活。
