Nasr Ali M, Mortagi Yasmin I, Elwahab Nashwa H Abd, Alfaifi Mohammad Y, Shati Ali A, Elbehairi Serag Eldin I, Elshaarawy Reda F M, Kamal Islam
Department of Pharmaceutics, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt.
Department of Pharmaceutics, Faculty of Pharmacy, Sinai University, Alarish 45511, Egypt.
Pharmaceutics. 2022 Jun 25;14(7):1350. doi: 10.3390/pharmaceutics14071350.
(1) Background: L. (thyme) essential oil (TEO) has gained much attention because of its long history of medicinal usage. However, the lack of precise chemical profiling of the TEO and methods to optimize the bioactivity and delivery of its constituents has hampered its research on quality control and biological function; (2) Methods: The current study aimed to analyze the TEO's chemical composition using the GC-MS method and identify its key components. Another objective of this work is to study the impact of the protective layer of amphiphilic oligochitosan (AOC) on the physicochemical stability and transdermal potentials of TEO multilayer nanoemulsions formulated by the incorporation of TEO, Tween80, lecithin (Lec), and AOC; (3) Results: The AOC protective layer significantly improved the stability of TEO-based NEs as revealed by the constancy of their physicochemical properties (particle size and zeta potential) during storage for a week. Excessive fine-tuning of thyme extract NEs and the AOC protective layer's persistent positive charge have been contributed to the thyme extract's improved anti-inflammatory, transdermal, and anti-melanoma potentials; (4) Conclusions: the AOC-coated NEs could offer novel multifunctional nanoplatforms for effective transdermal delivery of lipophilic bioactive materials.
(1) 背景：百里香精油（TEO）因其悠久的药用历史而备受关注。然而，缺乏对TEO精确的化学剖析以及优化其成分生物活性和递送的方法，阻碍了其在质量控制和生物学功能方面的研究；(2) 方法：本研究旨在采用气相色谱 - 质谱联用（GC - MS）方法分析TEO的化学成分并鉴定其关键成分。这项工作的另一个目标是研究两亲性低聚壳聚糖（AOC）保护层对通过掺入TEO、吐温80、卵磷脂（Lec）和AOC制备的TEO多层纳米乳液的物理化学稳定性和透皮潜力的影响；(3) 结果：AOC保护层显著提高了基于TEO的纳米乳液的稳定性，这在其储存一周期间的物理化学性质（粒径和zeta电位）恒定中得到体现。百里香提取物纳米乳液的过度微调以及AOC保护层持续的正电荷有助于提高百里香提取物的抗炎、透皮和抗黑色素瘤潜力；(4) 结论：AOC包被的纳米乳液可为亲脂性生物活性材料的有效透皮递送提供新型多功能纳米平台。
