一个双顺反子亚基因组mRNA编码戊型肝炎病毒的ORF2和ORF3蛋白。
A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus.
作者信息
Graff Judith, Torian Udana, Nguyen Hanh, Emerson Suzanne U
机构信息
Molecular Hepatitis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-8009, USA.
出版信息
J Virol. 2006 Jun;80(12):5919-26. doi: 10.1128/JVI.00046-06.
Abstract
Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.
摘要
将含有从开放阅读框（ORF）2和3表达的新霉素抗性基因的戊型肝炎病毒复制子转染到Huh-7细胞中，并通过持续暴露于硫酸G418来选择含有功能性复制子的稳定细胞系。Northern印迹分析检测到全长复制子RNA和单个亚基因组RNA。这种加帽的亚基因组RNA在ORF3中前两个甲硫氨酸密码子下游的核苷酸5122处起始，并且是双顺反子的；不同阅读框中的两个紧密间隔的甲硫氨酸密码子用于ORF3和ORF2翻译的起始。
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