Boija Elisabet, Johansson Gunnar
Department of Biochemistry and Organic Chemistry, Biomedical Center, Uppsala University, Box 576, SE-751 23 Uppsala, Sweden.
Biochim Biophys Acta. 2006 May;1758(5):620-6. doi: 10.1016/j.bbamem.2006.04.007. Epub 2006 Apr 21.
In order to elucidate the modes of interaction between lignin precursors and membranes, we have studied the influence of temperature, lipid composition and buffer composition on the partitioning of monolignol and dilignol model substances into phospholipid bilayers. The partitioning was determined by immobilized liposome chromatography, which is an established method for studies of pharmaceutical drugs but a new approach in studies of lignin synthesis. The temperature dependence of the retention and the effect of a high ammonium sulfate concentration in the mobile phase demonstrated that the interaction involved both hydrophobic effects and polar interactions. There was also a good correlation between the partitioning and the estimated hydrophobicity, in terms of octanol/water partitioning. The partitioning behavior of the model substances suggests that passive diffusion over the cell membrane is a possible transport route for lignin precursors. This conclusion is strengthened by comparison of the present results with the partitioning of pharmaceutical drugs that are known to pass cell membranes by diffusion.
为了阐明木质素前体与膜之间的相互作用模式，我们研究了温度、脂质组成和缓冲液组成对单木质醇和二木质醇模型物质在磷脂双层中分配的影响。分配通过固定化脂质体色谱法测定，这是一种用于研究药物的既定方法，但在木质素合成研究中是一种新方法。保留的温度依赖性以及流动相中高硫酸铵浓度的影响表明，相互作用涉及疏水作用和极性相互作用。就辛醇/水分配而言，分配与估计的疏水性之间也存在良好的相关性。模型物质的分配行为表明，细胞膜上的被动扩散是木质素前体的一种可能运输途径。通过将目前的结果与已知通过扩散穿过细胞膜的药物的分配进行比较，这一结论得到了加强。
