Tang Wenting, Pu Chuanfen, Li Man
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
PLoS One. 2017 Jan 4;12(1):e0164594. doi: 10.1371/journal.pone.0164594. eCollection 2017.
Liposomes constructed from Escherichia coli membrane lipids were used as a pseudo-stationary phase in capillary electrophoresis and immobilised liposome chromatography to evaluate the interaction between antibacterial peptide (ABP) Apep10 and bacterial membrane lipids. The peptide mobility decreased as the concentration of liposomes increased, providing evidence for the existence of this interaction. The binding constant between Apep10 and the Escherichia coli membranes lipid liposome was higher than that of Apep10 with a mixed phospholipids liposome at the same temperature. The capillary electrophoresis results indicate that the binding ability of Apep10 with a liposome was dependent on the liposome's lipid compositions. Thermodynamic analysis by immobilised liposome chromatography indicated that hydrophobic and electrostatic effects contributed to the partitioning of Apep10 in the membrane lipids. The liposomes constructed from bacterial membrane lipid were more suitable as the model membranes used to study dynamic ABP/membrane interactions than those constructed from specific ratios of particular phospholipids, with its more biomimetic phospholipid composition and contents. This study provides an appropriate model for the evaluation of ABP-membrane interactions.
由大肠杆菌膜脂构建的脂质体被用作毛细管电泳和固定化脂质体色谱中的假固定相，以评估抗菌肽（ABP）Apep10与细菌膜脂之间的相互作用。随着脂质体浓度的增加，肽的迁移率降低，这为这种相互作用的存在提供了证据。在相同温度下，Apep10与大肠杆菌膜脂脂质体之间的结合常数高于Apep10与混合磷脂脂质体之间的结合常数。毛细管电泳结果表明，Apep10与脂质体的结合能力取决于脂质体的脂质组成。固定化脂质体色谱的热力学分析表明，疏水作用和静电作用有助于Apep10在膜脂中的分配。由细菌膜脂构建的脂质体比由特定比例的特定磷脂构建的脂质体更适合作为研究ABP与膜动态相互作用的模型膜，因为其磷脂组成和含量更具仿生学特性。本研究为评估ABP与膜的相互作用提供了一个合适的模型。
