α-甲基辅酶A消旋酶在肾细胞癌中的表达

Alpha-methyl CoA racemase expression in renal cell carcinomas.

作者信息

Molinié Vincent, Balaton André, Rotman Samuel, Mansouri Douha, De Pinieux Isabelle, Homsi Toufik, Guillou Louis

机构信息

Department of Pathology, Hôpital Saint Joseph, 75014 Paris Cedex, France.

出版信息

Hum Pathol. 2006 Jun;37(6):698-703. doi: 10.1016/j.humpath.2006.01.012.

DOI:10.1016/j.humpath.2006.01.012

PMID:16733210

Abstract

Alpha-methyl CoA racemase (AMACR), a new molecular marker for prostate cancer, has been recently reported to be one of the most highly expressed genes in papillary renal cell carcinomas (RCCs). We tested the diagnostic usefulness of AMACR antibody in a series of 110 renal tumors: 53 papillary RCCs (33 type 1, 20 type 2); 25 conventional RCCs; 6 chromophobe RCCs; 9 oncocytomas; 5 mucinous tubular and spindle tumors; 2 urothelial carcinomas; 7 angiomyolipomas; and 2 Bellini carcinomas. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections, with a primary prediluted rabbit monoclonal anti-AMACR antibody. Both type 1 and type 2 papillary RCCs exhibited cytoplasmic immunoreactivity for AMACR, with diffuse strong granular staining in 96.4% (53/55) of tumors, without correlation with type or nuclear grade. The 5 mucinous, tubular, and spindle cell carcinomas strongly expressed AMACR, and only 5 of 25 clear cell RCCs and 1 of 9 oncocytomas were focally reactive. The remaining 6 chromophobe RCCs, 5 urothelial carcinomas, and Bellini duct carcinomas showed no immunoreactivity for AMACR. Because high expression of AMACR is found in papillary RCCs (type 1 and 2) and in mucinous, tubular, and spindle cell carcinomas of the kidney, immunostaining for AMACR should be used in conjunction with other markers when histological typing of a renal tumor is difficult.

摘要

α-甲基辅酶A消旋酶（AMACR）是一种新型前列腺癌分子标志物，最近有报道称它是乳头状肾细胞癌（RCC）中表达水平最高的基因之一。我们检测了AMACR抗体在110例肾肿瘤中的诊断效用，其中包括53例乳头状RCC（33例1型，20例2型）；25例传统型RCC；6例嫌色性RCC；9例嗜酸细胞瘤；5例黏液性管状和梭形细胞瘤；2例尿路上皮癌；7例血管平滑肌脂肪瘤；以及2例贝氏管癌。采用预稀释的兔单克隆抗AMACR抗体对福尔马林固定、石蜡包埋的组织切片进行免疫组织化学染色。1型和2型乳头状RCC均显示出AMACR的细胞质免疫反应性，96.4%（53/55）的肿瘤呈弥漫性强颗粒状染色，与肿瘤类型或核分级无关。5例黏液性、管状和梭形细胞癌强烈表达AMACR，25例透明细胞RCC中只有5例以及9例嗜酸细胞瘤中有1例呈局灶性反应。其余6例嫌色性RCC、5例尿路上皮癌和贝氏管癌对AMACR无免疫反应性。由于在乳头状RCC（1型和2型）以及肾黏液性、管状和梭形细胞癌中发现AMACR高表达，因此在肾肿瘤组织学分型困难时，AMACR免疫染色应与其他标志物联合使用。