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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Roig T, Vinardell M P

Department de Ciències Fisiologiques Humanes i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.

Comp Biochem Physiol A Comp Physiol. 1991;98(1):3-7. doi: 10.1016/0300-9629(91)90568-w.

Intestinal absorption can be studied by in vitro and in vivo techniques. Among the in vivo ones, intestinal perfusion is the one more employed. 2. Intestinal perfusion could be performed by a simple perfusion of an intestinal segment or by a double perfusion of the intestine and the vascular bed simultaneously. 3. The double perfusion has the advantage of measuring the substrate appearance in the vascular circuit. 4. In this review we compare the different techniques described in the literature, paying attention to their advantages. 5. The best method is the one that maintains the animal alive throughout the experiment, because it provides information about intestinal absorption under conditions similar to the natural ones.

肠道吸收可以通过体外和体内技术进行研究。在体内技术中，肠道灌注是应用较多的一种。2. 肠道灌注可以通过简单地灌注一段肠段或同时对肠道和血管床进行双重灌注来进行。3. 双重灌注的优点是能够测量血管回路中底物的出现情况。4. 在本综述中，我们比较了文献中描述的不同技术，并关注它们的优点。5. 最好的方法是在整个实验过程中使动物保持存活的方法，因为它能在类似于自然条件的情况下提供有关肠道吸收的信息。

Roig T, Vinardell M P

Department de Ciències Fisiologiques Humanes i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.

Comp Biochem Physiol A Comp Physiol. 1991;98(1):3-7. doi: 10.1016/0300-9629(91)90568-w.

Intestinal absorption can be studied by in vitro and in vivo techniques. Among the in vivo ones, intestinal perfusion is the one more employed. 2. Intestinal perfusion could be performed by a simple perfusion of an intestinal segment or by a double perfusion of the intestine and the vascular bed simultaneously. 3. The double perfusion has the advantage of measuring the substrate appearance in the vascular circuit. 4. In this review we compare the different techniques described in the literature, paying attention to their advantages. 5. The best method is the one that maintains the animal alive throughout the experiment, because it provides information about intestinal absorption under conditions similar to the natural ones.

肠道吸收可以通过体外和体内技术进行研究。在体内技术中，肠道灌注是应用较多的一种。2. 肠道灌注可以通过简单地灌注一段肠段或同时对肠道和血管床进行双重灌注来进行。3. 双重灌注的优点是能够测量血管回路中底物的出现情况。4. 在本综述中，我们比较了文献中描述的不同技术，并关注它们的优点。5. 最好的方法是在整个实验过程中使动物保持存活的方法，因为它能在类似于自然条件的情况下提供有关肠道吸收的信息。