Sahin Mehmet, Cam Hakan, Olgar Seref, Tunc Sevket Ercan, Arslan Cagatay, Uz Efkan, Yilmaz H Ramazan
Department of Internal Medicine, School of Medicine, Suleyman Demirel University, Isparta, Turkey.
Mol Cell Biochem. 2006 Oct;291(1-2):155-60. doi: 10.1007/s11010-006-9209-4. Epub 2006 May 30.
Drug-induced liver toxicity is a common cause of liver injury. This study was designed to elucidate whether high dose vancomycin (VCM) induces oxidative stress in liver and to investigate the protective effects of erdosteine, an expectorant agent. Twenty-two young Wistar rats were divided into three groups as follows: control group, VCM, and VCM plus erdosteine. VCM was administered intraperitoneally in the dosage of 200 mg/kg twice daily for 7 days. Erdosteine was administered orally administered once a day at a dose of 10 mg/kg body weight. The activities of antioxidant enzymes such as superoxide dismutase and catalase as well as the concentration of malondialdehyde, as an indicator of lipid peroxidation, were measured to evaluate oxidative stress in homogenates of the liver. VCM administration increased malondialdehyde levels (p < 0.001), superoxide dismutase (p < 0.01) and catalase (p < 0.001) activities. Erdosteine co-administration with VCM injections caused significantly decreased malondialdehyde levels (p < 0.001), superoxide dismutase (p < 0.01) and catalase (p < 0.001) activities in liver tissue when compared with VCM alone. It can be concluded that erdosteine may prevent VCM-induced oxidative changes in liver by reducing reactive oxygen species.
药物性肝毒性是肝损伤的常见原因。本研究旨在阐明高剂量万古霉素（VCM）是否会在肝脏中诱导氧化应激，并研究祛痰剂厄多司坦的保护作用。将22只年轻的Wistar大鼠分为三组：对照组、VCM组和VCM加厄多司坦组。VCM以200mg/kg的剂量腹腔注射，每天两次，共7天。厄多司坦以10mg/kg体重的剂量每天口服一次。测量抗氧化酶如超氧化物歧化酶和过氧化氢酶的活性以及丙二醛的浓度（作为脂质过氧化的指标），以评估肝脏匀浆中的氧化应激。给予VCM会增加丙二醛水平（p<0.001）、超氧化物歧化酶（p<0.01）和过氧化氢酶（p<0.001）的活性。与单独使用VCM相比，厄多司坦与VCM联合注射可使肝组织中的丙二醛水平（p<0.001）、超氧化物歧化酶（p<0.01）和过氧化氢酶（p<0.001）活性显著降低。可以得出结论，厄多司坦可能通过减少活性氧来预防VCM诱导的肝脏氧化变化。
