Fu Di, Guo Qulian, Ai Yuhang, Cai Hongwei, Yan Jianqin, Dai Ruping
Department of Anesthesiology, Xiang-Ya Hospital, Xiang-Ya College of Medicine, Central South University, Changsha, Hunan, China.
Neurochem Res. 2006 Mar;31(3):333-40. doi: 10.1007/s11064-005-9032-4. Epub 2006 May 3.
The present study investigated the expression patterns of glial cells and interleukin-1beta (IL-1beta) in the rat spinal cord after a surgical incision, which is closely related with clinical postoperative pain. Microglia and astrocytes became activated in the spinal cord following incision. Real-time polymerase chain reaction (PCR) and immunohistochemisty showed that IL-1beta mRNA and protein level in the spinal cord was transiently upregulated after surgical incision. The increased IL-1beta-immunoreactivity (IR) was mainly localized in neurons but not the activated microglia or astrocytes. Although obvious increase in IL-1beta-IR could be observed in the lumbar segments of the spinal cord ipsilateral to a hind paw incision, significant upregulation of IL-1beta was not detected in the lumbar segments following thoracic incision. The present study indicated that surgical incision could induce glial activation and segmental upregulation of IL-1beta in the spinal cord. The activated glial cells and upregulated IL-1beta, in turn, may be involved in the incision-induced pain hypersensitivity.
本研究调查了手术切口后大鼠脊髓中胶质细胞和白细胞介素-1β（IL-1β）的表达模式，手术切口与临床术后疼痛密切相关。切口后脊髓中的小胶质细胞和星形胶质细胞被激活。实时聚合酶链反应（PCR）和免疫组织化学显示，手术切口后脊髓中IL-1β的mRNA和蛋白水平短暂上调。IL-1β免疫反应性（IR）增加主要定位于神经元，而非激活的小胶质细胞或星形胶质细胞。尽管在后爪切口同侧脊髓腰段可观察到IL-1β-IR明显增加，但胸段切口后腰段未检测到IL-1β的显著上调。本研究表明，手术切口可诱导脊髓中胶质细胞激活和IL-1β的节段性上调。激活的胶质细胞和上调的IL-1β反过来可能参与切口诱导的疼痛超敏反应。
