Feng Shan-wei, Zhang Cheng, Yao Xiao-li, Yu Mei-juan, Li Jing-lun, Chen Song-lin, Liu Tai-yun, Lu Xi-lin
Department of Neurology, the First Affiliated Hospital, SUN Yat-sen University, Guangzhou 510080, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Apr;28(2):178-81.
To investigate the dynamic changes of dystrophin expression in mdx mice after bone marrow stem cells transplantation.
The bone marrow stem cells of C57 BL/6 mice (aged 6 to 8 weeks) were injected intravenously into the mdx mice (aged 7 to 9 weeks), which were preconditioned with 7Gy gamma ray. The amount of dystrophin;expression in gastrocnemius was detected by immunofluorescence, reverse transcription-polymerase chain reaction and Western blot at week 5, 8, 12 and 16 after transplantation.
At week 5 after bone marrow stem cells transplantation, the dystrophin expression detected in mdx mice were very low; however, its expression increased along with time. At week 16 week, about 12% muscle cells of all transplanted mice expressed dystrophin. There were less centrally placed myonuclei than the control mdx mice, whereas peripheral myonuclei increased.
After having been injected into mdx mice, the allogenic bone marrow stem cells have a trend to reach the injured muscle tissues and differentiate to fibers that can express dystrophin and the expression increased with time. The bone marrow stem cells participates in the repair and regeneration of the injured tissues permanently and constantly.
研究骨髓干细胞移植后mdx小鼠中抗肌萎缩蛋白表达的动态变化。
将6至8周龄C57 BL/6小鼠的骨髓干细胞静脉注射到经7Gyγ射线预处理的7至9周龄mdx小鼠体内。在移植后第5、8、12和16周，通过免疫荧光、逆转录-聚合酶链反应和蛋白质免疫印迹法检测腓肠肌中抗肌萎缩蛋白的表达量。
骨髓干细胞移植后第5周，mdx小鼠中检测到的抗肌萎缩蛋白表达非常低；然而，其表达随时间增加。在第16周，所有移植小鼠中约12%的肌细胞表达抗肌萎缩蛋白。与对照mdx小鼠相比，中央核仁较少，而周边核仁增加。
同种异体骨髓干细胞注入mdx小鼠后，有趋向于到达受损肌肉组织并分化为可表达抗肌萎缩蛋白的纤维，且表达随时间增加。骨髓干细胞永久性且持续地参与受损组织的修复和再生。
