Yu M, Zhang C, Zhang Y, Feng S, Yao X, Lu X
Department of Neurology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Cytotherapy. 2007;9(1):44-52. doi: 10.1080/14653240601114815.
The value of transplantation of BM stem cells in aged (12-month-old) mdx was evaluated because it is thought to be a more ideal model for studying the praxiology of Duchenne muscular dystrophy (DMD). The possible mechanisms of stem cell differentiation were then discussed.
BM was isolated from 8-10-week-old male C57 BL/10 mice. After injecting BM cells into 12-month-old female mdx mice through the tail vein, the expression of dystrophin and MyoD was detected at different time points by immunofluorescence staining, RT-PCR and Western blot.
The C57 male mice donor-specific and Y-chromosome-specific sequence could be detected in all female aged mdx mice, implying the success of the transplantation. Expression of dystrophin and MyoD was detected and increased over time.
BM cells were recruited to the muscle and partially restored specific pathophysiologic features of the dystrophic muscle in aged mdx mice. Muscle differentiation of BM cells recapitulated embryonic myogenesis.
评估了骨髓干细胞移植在老年（12个月大）mdx小鼠中的价值，因为它被认为是研究杜氏肌营养不良症（DMD）行为学的更理想模型。随后讨论了干细胞分化的可能机制。
从8 - 10周龄雄性C57 BL/10小鼠中分离骨髓。通过尾静脉将骨髓细胞注射到12个月大的雌性mdx小鼠体内后，在不同时间点通过免疫荧光染色、RT-PCR和蛋白质印迹法检测抗肌萎缩蛋白和肌分化抗原（MyoD）的表达。
在所有老年雌性mdx小鼠中都能检测到C57雄性小鼠供体特异性和Y染色体特异性序列，这意味着移植成功。检测到抗肌萎缩蛋白和MyoD的表达，且随时间增加。
骨髓细胞被募集到肌肉中，并部分恢复了老年mdx小鼠营养不良性肌肉的特定病理生理特征。骨髓细胞的肌肉分化重现了胚胎期的肌发生过程。
