Lozano M T, Garcia Ayala A, Abad M E, Agulleiro B
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
Gen Comp Endocrinol. 1991 Feb;81(2):198-206. doi: 10.1016/0016-6480(91)90004-p.
Insulin (INS)- and somatostatin (SST)-immunoreactive cells were demonstrated by light immunocytochemistry in the endocrine pancreas of sea bass (Dicentrarchus labrax). INS-immunoreactive cells were identified using bovine/porcine, bonito, and salmon (s) INS antisera; the immunostaining was abolished when each antiserum was preabsorbed with its respective peptide but not with unrelated peptides. These cells also reacted with mammal (m) SST-28 (4-14) antiserum. The immunoreaction did not change when this antiserum was preabsorbed by bovine INS. INS-immunoreactive cells were located in the central part of the endocrine areas of the principal, intermediate, and small islets. Two SST-immunoreactive cell types (D1 and D2) were revealed. D1 cells, immunoreactive to SST 14 (562) and sSST-25 antisera, were located next to the glucagon-immunoreactive cells in the peripheral part of the endocrine areas. D2 cells, immunoreactive to SST-14 (562), SST-14 (566), and mSST-28 (4-14) antisera, were found in apposition to the INS-immunoreactive cells. The specificity controls showed that D1 cells expressed sSST-25-like peptides, while D2 cells might contain SST-14 and/or mSST-28-like peptides. The close topographic association between the different SST-immunoreactive cells and both glucagon- and insulin-immunoreactive cells might indicate the existence of a specific paracrine regulation of each endocrine cell type in the sea bass endocrine pancreas.
通过光免疫细胞化学方法在海鲈（欧洲鲈）的内分泌胰腺中证实了胰岛素（INS）和生长抑素（SST）免疫反应性细胞。使用牛/猪、鲣鱼和鲑鱼（s）INS抗血清鉴定INS免疫反应性细胞；当每种抗血清用其各自的肽预先吸附时，免疫染色被消除，但用无关肽预先吸附时则不会。这些细胞也与哺乳动物（m）SST - 28（4 - 14）抗血清发生反应。当该抗血清用牛INS预先吸附时，免疫反应没有变化。INS免疫反应性细胞位于主胰岛、中间胰岛和小胰岛内分泌区域的中央部分。揭示了两种SST免疫反应性细胞类型（D1和D2）。对SST 14（562）和sSST - 25抗血清有免疫反应的D1细胞位于内分泌区域周边的胰高血糖素免疫反应性细胞旁边。对SST - 14（562）、SST - 14（566）和mSST - 28（4 - 14）抗血清有免疫反应的D2细胞与INS免疫反应性细胞相邻。特异性对照表明，D1细胞表达sSST - 25样肽，而D2细胞可能含有SST - 14和/或mSST - 28样肽。不同SST免疫反应性细胞与胰高血糖素和胰岛素免疫反应性细胞之间紧密的地形学关联可能表明在海鲈内分泌胰腺中每种内分泌细胞类型存在特定的旁分泌调节。
