Lozano M T, Garcia Ayala A, Abad M E, Agulleiro B
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
Gen Comp Endocrinol. 1991 Feb;81(2):187-97. doi: 10.1016/0016-6480(91)90003-o.
PP-, PYY-, and glucagon-immunoreactive cells were immunocytochemically identified in the pancreatic islets of Dicentrarchus labrax (sea bass). PYY cells also reacted with anti-PP serum. The specificity control showed that preabsorption of PP antiserum by PYY peptide abolished the immunostaining, while the reaction did not change when the PYY antiserum was preabsorbed by PP. These results suggested the existence of a PP/PYY molecule in the sea bass islets. The islet distribution of PP/PYY-immunoreactive cells differed markedly. Thus, in the principal islet and some intermediate islets few PP/PYY-immunoreactive cells are present (type I islets), whereas in the smaller and some intermediate ones they are numerous (type II islets). Adjacent sections stained by peroxidase-antiperoxidase (PAP) technique and individual sections stained by immunofluorescence double staining showed the coexistence of glucagon and PP/PYY-like immunoreactivities. Both islet types contained cells with PP/PYY coexisting with glucagon peptide, while cells showing solely glucagon immunoreactivity were found in type I islets only.
在欧洲海鲈的胰岛中，通过免疫细胞化学方法鉴定出了胰多肽（PP）、肽YY（PYY）和胰高血糖素免疫反应性细胞。PYY细胞也与抗PP血清发生反应。特异性对照显示，用PYY肽预吸收PP抗血清可消除免疫染色，而用PP预吸收PYY抗血清时反应不变。这些结果表明欧洲海鲈胰岛中存在PP/PYY分子。PP/PYY免疫反应性细胞的胰岛分布明显不同。因此，在主胰岛和一些中间胰岛中，PP/PYY免疫反应性细胞很少（I型胰岛），而在较小的胰岛和一些中间胰岛中它们很多（II型胰岛）。用过氧化物酶-抗过氧化物酶（PAP）技术染色的相邻切片以及用免疫荧光双重染色的单个切片显示胰高血糖素和PP/PYY样免疫反应性共存。两种胰岛类型都含有PP/PYY与胰高血糖素肽共存的细胞，而仅显示胰高血糖素免疫反应性的细胞仅在I型胰岛中发现。
