Kaptaner Burak
University of Van Yuzuncu Yil, Faculty of Science, Department of Biology.
Eur J Histochem. 2019 Feb 25;63(1):2999. doi: 10.4081/ejh.2019.2999.
The Lake Van fish (Alburnus tarichi) is a species that is endemic to Turkey's Lake Van basin. In this study, the regional distribution, volume density, and relative frequency of some pancreatic endocrine cells in Lake Van fish were investigated via immunohistochemistry using specific mammalian antibodies. The pancreatic tissue was observed to be surrounded by adipose tissue, which was adjacent to the gall bladder or extrahepatic bile duct, or dispersed in the adipose tissue ranked among coils of post-esophageal swelling and intestine. The pancreatic endocrine cells were examined, including the islets, exocrine pancreas, and pancreatic ducts. According to the modified aldehyde fuchsin staining and immunohistochemistry, insulin-secreting beta cells were observed to localize throughout the islets. Glucagon immune-reactive (IR) cells were observed to be situated moderately on the islet periphery, and were rarely determined in the islet central region. A small number of somatostatin-IR cells were observed in the islet centers and peripheries. Similar distributions of those 3 endocrine cells were also determined in the secondary islets. Additionally, the endocrine cell percentages did not differ between the primary and secondary islets; insulin-, glucagon- and somatostatin-IR cells comprised approximately 54%, 29%, and 11% of the endocrine cells in the principal islets, whereas they comprised 52%, 27%, and 14% in the secondary islets, respectively. Insulin-, glucagon- and somatostatin-IR cells were also determined among the epithelium and subepithelial connective tissue in the pancreatic ducts or exocrine areas of the pancreas. With this study, the existence, regional distribution, and relative frequency of the insulin-, glucagon- and somatostatin-IR cells were first investigated in the pancreatic tissue of Lake Van fish and the results were discussed.
凡湖鱼（塔尔氏欧白鱼）是土耳其凡湖流域特有的一种鱼类。在本研究中，使用特定的哺乳动物抗体，通过免疫组织化学方法研究了凡湖鱼某些胰腺内分泌细胞的区域分布、体积密度和相对频率。观察到胰腺组织被脂肪组织包围，脂肪组织与胆囊或肝外胆管相邻，或分散在食管后肿胀和肠道盘绕处的脂肪组织中。对胰腺内分泌细胞进行了检查，包括胰岛、外分泌胰腺和胰管。根据改良的醛复红染色和免疫组织化学方法，观察到分泌胰岛素的β细胞遍布整个胰岛。胰高血糖素免疫反应（IR）细胞适度位于胰岛周边，在胰岛中央区域很少发现。在胰岛中心和周边观察到少量生长抑素-IR细胞。在次级胰岛中也确定了这3种内分泌细胞的类似分布。此外，初级和次级胰岛之间的内分泌细胞百分比没有差异；胰岛素、胰高血糖素和生长抑素-IR细胞在主要胰岛的内分泌细胞中分别约占54%、29%和11%，而在次级胰岛中分别占52%、27%和14%。在胰腺导管或胰腺外分泌区域的上皮和上皮下结缔组织中也确定了胰岛素、胰高血糖素和生长抑素-IR细胞。通过本研究，首次对凡湖鱼胰腺组织中胰岛素、胰高血糖素和生长抑素-IR细胞的存在、区域分布和相对频率进行了研究，并对结果进行了讨论。
