Halatsi Konstantia, Oikonomou Ioannis, Lambiri Maria, Mandilara Georgia, Vatopoulos Alkiviadis, Kyriacou Adamantini
Department of Dietetics and Nutritional Science, Harokopio University, Athens, Greece.
FEMS Microbiol Lett. 2006 Jun;259(2):201-7. doi: 10.1111/j.1574-6968.2006.00266.x.
Bacteria communicate with one another and with their host using chemical signalling molecules. This phenomenon is generally described as quorum sensing. A set of primers for PCR detection of Salmonella spp. has been designed using as target the sdiA gene which encodes a signal receptor of the LuxR family. The PCR product (274 bp) was confirmed by sequencing. A number of 81 non-Salmonella strains (representing 24 different species) were tested and gave negative results, while a total of 101 different serotypes of Salmonella (155 strains) tested positive for the presence of the sdiA gene. The sensitivity and specificity of the sdiA-based PCR assay were also checked in artificially contaminated human faecal samples. In this study, we demonstrate that quorum sensing genes can be successfully exploited as diagnostic markers.
细菌利用化学信号分子相互之间以及与宿主进行交流。这种现象通常被描述为群体感应。已经设计了一组用于PCR检测沙门氏菌属的引物，其靶标是编码LuxR家族信号受体的sdiA基因。PCR产物（274 bp）经测序确认。对81株非沙门氏菌菌株（代表24个不同物种）进行了测试，结果均为阴性，而总共101种不同血清型的沙门氏菌（155株）检测到sdiA基因呈阳性。还在人工污染的人类粪便样本中检查了基于sdiA的PCR检测方法的灵敏度和特异性。在本研究中，我们证明群体感应基因可成功用作诊断标志物。
