Rancan Milena, Rossi Simona, Sabatini Anna Gloria
Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Istituto Nazionale di Apicoltura, Via di Saliceto 80, 40128 Bologna, Italy.
J Chromatogr A. 2006 Aug 4;1123(1):60-5. doi: 10.1016/j.chroma.2006.05.006. Epub 2006 Jun 2.
A method for the determination of Thiamethoxam in bee samples was set up by means of high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor (HPLC-h nu-ED). Analytical method was based on a rapid sample extraction procedure with acetone, followed by chromatographic separation into a C18-RP column isocratically operated by 60 mM phosphate buffer/acetonitrile (75/25) mobile phase at pH 2.7. A photochemical reactor was used as a tool to verify and eventually quantify the presence of Thiamethoxam in the samples by distinguishing it from interference contribution. Detection was performed with a potential of 880 mV after a photoactivation with a 254 nm light. The least detectable dose was 0.002 mg kg(-1). Recovery rates ranged between 59.88 and 71.62%.
建立了一种采用高效液相色谱-电化学检测器-柱后光化学反应器（HPLC-hν-ED）测定蜜蜂样本中噻虫嗪的方法。分析方法基于用丙酮进行快速样品萃取程序，随后在C18反相柱上进行色谱分离，该柱由pH 2.7的60 mM磷酸盐缓冲液/乙腈（75/25）流动相等度洗脱。使用光化学反应器作为工具，通过区分噻虫嗪与干扰物的贡献来验证并最终定量样品中噻虫嗪的存在。在用254 nm光进行光活化后，在880 mV的电位下进行检测。最低检测剂量为0.002 mg kg⁻¹。回收率在59.88%至71.62%之间。
