College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 May;26(5):713-8. doi: 10.1080/02652030802672638.
An enzyme-linked immunosorbent assay (ELISA) was used for the determination of residues of imidacloprid and thiamethoxam insecticides in honey after simple dilution of the samples without either extraction or cleanup. The ELISA enabled accurate determination of imidacloprid and thiamethoxam down to limits of 20 and 5 ng g(-1) in honey, respectively. Average recoveries of imidacloprid and thiamethoxam from the fortified honey samples were 90-120 and 96-122%, and coefficients of variation ranged 5-12 and 3-15%, respectively. The results from the ELISA agreed well with those by liquid chromatography-mass spectrometry (LC-MS) for the insecticide-spiked samples, with a correlation coefficient (r(2)) of 0.96 and a regression coefficient (slope) of 1.03. The results indicate that ELISA is a suitable tool for the quantification of imidacloprid and thiamethoxam in honey.
采用酶联免疫吸附测定法（ELISA），对未经提取或净化的样品进行简单稀释，即可测定蜂蜜中残留的吡虫啉和噻虫嗪杀虫剂。ELISA 可分别准确测定蜂蜜中吡虫啉和噻虫嗪的含量，检测限分别为 20 和 5ng/g。从加标蜂蜜样品中测得的吡虫啉和噻虫嗪的平均回收率分别为 90-120%和 96-122%，变异系数分别为 5-12%和 3-15%。ELISA 的检测结果与经 LC-MS 检测的杀虫剂加标样品的结果吻合良好，相关系数（r(2)）为 0.96，回归系数（斜率）为 1.03。结果表明，ELISA 是一种适用于定量检测蜂蜜中吡虫啉和噻虫嗪的方法。
