DNA-碱性多肽相互作用的特异性。掺入聚赖氨酸和聚精氨酸中的中性残基的影响。
Specifity of DNA-basic polypeptide interactions. Influence of neutral residues incorporated into polylysine and polyarginine.
作者信息
Wehling K, Arfmann H A, Standke K H, Wagner K G
出版信息
Nucleic Acids Res. 1975 Jun;2(6):799-807. doi: 10.1093/nar/2.6.799.
Abstract
An approach is described for evaluation of the specificity of basic polypeptides concerning the base pair composition of DNA. The polypeptides were covalently bound to CNBr activated agarose and two DNAs strongly different in base composition but of equal molecular weight were loaded and detached by a NaCl gradient. The difference in the NaCl concerntrations between the elution maxima of the two DNAs was taken as a measure for the recognition specificity. The results obtained confirmed the known AT- und GC-specificity of polylysine and polyarginine, respectively. Neutral residues incorporated into polylysine generally reduce the interaction affinity and also the AT-specificity of their host. This behavior is very pronounced with three homogeneous fractions of clupeine containing about one third of neutral aliphatic amino acids within clusters of arginine; the base pair specificity of these arginine copolymers was found to be practically nil.
摘要
本文描述了一种评估碱性多肽与DNA碱基对组成特异性的方法。将多肽共价结合到溴化氰活化的琼脂糖上，加载两种碱基组成差异很大但分子量相等的DNA，并通过NaCl梯度使其分离。将两种DNA洗脱峰最大值之间的NaCl浓度差异作为识别特异性的指标。所得结果分别证实了聚赖氨酸和聚精氨酸已知的AT特异性和GC特异性。掺入聚赖氨酸中的中性残基通常会降低其相互作用亲和力及其宿主的AT特异性。这种行为在三种鲱精蛋白均一馏分中非常明显，这些馏分在精氨酸簇中含有约三分之一的中性脂肪族氨基酸；发现这些精氨酸共聚物的碱基对特异性几乎为零。
相似文献
1
Specifity of DNA-basic polypeptide interactions. Influence of neutral residues incorporated into polylysine and polyarginine.DNA-碱性多肽相互作用的特异性。掺入聚赖氨酸和聚精氨酸中的中性残基的影响。
Nucleic Acids Res. 1975 Jun;2(6):799-807. doi: 10.1093/nar/2.6.799.
2
Specificity of DNA basic polypeptide interactions. II+ Influence of aromatic amino acid residues investigated with agarose bound lysine copolypeptides.DNA碱性多肽相互作用的特异性。II. 用琼脂糖结合赖氨酸共多肽研究芳香族氨基酸残基的影响。
Nucleic Acids Res. 1977 Mar;4(3):513-22. doi: 10.1093/nar/4.3.513.
3
Infrared linear dichroism investigations of deoxyribonucleic acid complexes with poly(L-arginine) and poly(L-lysine).
Biochemistry. 1975 Sep 23;14(19):4191-7. doi: 10.1021/bi00690a007.
4
[Interaction of cholesterol with polypeptides and amino acids].
Biokhimiia. 1982 Feb;47(2):226-32.
5
Interaction of poly(L-lysine) and copolymers of lysine with immobilized DNA.聚-L-赖氨酸及赖氨酸共聚物与固定化DNA的相互作用。
Arch Biochem Biophys. 1979 Mar;193(1):204-12. doi: 10.1016/0003-9861(79)90024-9.
6
Cellular Uptake Mechanism of Cationic Branched Polypeptides with Poly[l-Lys] Backbone.具有聚[l-赖氨酸]主链的阳离子支链多肽的细胞摄取机制
ACS Comb Sci. 2017 Apr 10;19(4):246-254. doi: 10.1021/acscombsci.6b00133. Epub 2017 Mar 9.
7
The preferential interactions of polylysine and polyarginine with specific base sequences in DNA.聚赖氨酸和聚精氨酸与DNA中特定碱基序列的优先相互作用。
Proc Natl Acad Sci U S A. 1966 Oct;56(4):1325-32. doi: 10.1073/pnas.56.4.1325.
8
Some properties of polylysine-accessible DNA.聚赖氨酸可及DNA的一些特性。
Cell Biol Int Rep. 1981 Feb;5(2):201-10. doi: 10.1016/0309-1651(81)90029-1.
9
Amino acid requirement for the high affinity binding of a selected arginine-rich peptide with the HIV Rev-response element RNA.一种选定的富含精氨酸的肽与HIV Rev反应元件RNA高亲和力结合所需的氨基酸。
J Pept Sci. 2008 Aug;14(8):924-35. doi: 10.1002/psc.1027.
10
Spin-label electron spin resonance studies on the interactions of lysine peptides with phospholipid membranes.自旋标记电子自旋共振研究赖氨酸肽与磷脂膜的相互作用。
Biophys J. 1997 Nov;73(5):2546-55. doi: 10.1016/S0006-3495(97)78283-3.
引用本文的文献
1
Non-Viral Nucleic Acid Delivery Strategies to the Central Nervous System.非病毒核酸向中枢神经系统的递送策略
Front Mol Neurosci. 2016 Nov 1;9:108. doi: 10.3389/fnmol.2016.00108. eCollection 2016.
2
Guanidinylated block copolymers for gene transfer: A comparison with amine-based materials for in vitro and in vivo gene transfer efficiency.用于基因传递的胍基化嵌段共聚物：与基于胺的材料在体外和体内基因传递效率方面的比较。
Biomaterials. 2015 Jun;54:87-96. doi: 10.1016/j.biomaterials.2015.03.008. Epub 2015 Mar 30.
3
The binding of protamines to DNA; role of protamine phosphorylation.鱼精蛋白与DNA的结合；鱼精蛋白磷酸化的作用。
Biophys Struct Mech. 1980;6(2):95-110. doi: 10.1007/BF00535747.
4
Recognition of base pairs by polar peptides in double stranded DNA.双链DNA中极性肽对碱基对的识别
Nucleic Acids Res. 1982 Mar 11;10(5):1707-20. doi: 10.1093/nar/10.5.1707.
5
Cooperative binding of fluorescein-labeled clupeine by DNA.荧光素标记的鲱精蛋白与DNA的协同结合
Nucleic Acids Res. 1976 Jan;3(1):149-64. doi: 10.1093/nar/3.1.149.
6
Phosphorylated protamines. II. Circular dichroism of complexes with DNA, dependency on ionic strength.磷酸化鱼精蛋白。II. 与DNA形成的复合物的圆二色性，对离子强度的依赖性。
Nucleic Acids Res. 1977 Jan;4(1):163-76. doi: 10.1093/nar/4.1.163.
7
Phosphorylated protamines. I. Binding stoichiometry and thermal stability of complexes in DNA.磷酸化鱼精蛋白。I. DNA中复合物的结合化学计量和热稳定性。
Nucleic Acids Res. 1977 Jan;4(1):149-62. doi: 10.1093/nar/4.1.149.
8
Specificity of DNA basic polypeptide interactions. II+ Influence of aromatic amino acid residues investigated with agarose bound lysine copolypeptides.DNA碱性多肽相互作用的特异性。II. 用琼脂糖结合赖氨酸共多肽研究芳香族氨基酸残基的影响。
Nucleic Acids Res. 1977 Mar;4(3):513-22. doi: 10.1093/nar/4.3.513.
本文引用的文献
1
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.根据热变性温度确定脱氧核糖核酸的碱基组成
J Mol Biol. 1962 Jul;5:109-18. doi: 10.1016/s0022-2836(62)80066-7.
2
3
On the interaction of nucleotides with poly-L-lysine and poly-L-arginine. I. The influence of the nucleotide base on the binding behavior.
Biochemistry. 1968 May;7(5):1771-7. doi: 10.1021/bi00845a022.
4
The preferential interactions of polylysine and polyarginine with specific base sequences in DNA.聚赖氨酸和聚精氨酸与DNA中特定碱基序列的优先相互作用。
Proc Natl Acad Sci U S A. 1966 Oct;56(4):1325-32. doi: 10.1073/pnas.56.4.1325.
5
[Studies on interactions between DNA and the alternating polypeptide (L-lysyl-L-ananyl)n].[DNA与交替多肽（L-赖氨酰-L-丙氨酰）n之间相互作用的研究]
Eur J Biochem. 1972 Mar 15;26(1):90-5. doi: 10.1111/j.1432-1033.1972.tb01743.x.
6
Deoxyribonucleic acid-polylysine complexes. Structure and nucleotide specificity.脱氧核糖核酸-聚赖氨酸复合物。结构与核苷酸特异性。
Biochemistry. 1969 Aug;8(8):3219-32. doi: 10.1021/bi00836a014.
7
Recognition of nucleotide sequences.核苷酸序列的识别
Annu Rev Biochem. 1969;38:841-80. doi: 10.1146/annurev.bi.38.070169.004205.
8
Protein-nucleic acid interactions. 3. Cation effect on binding strength and specificity.蛋白质-核酸相互作用。3. 阳离子对结合强度和特异性的影响。
Biochemistry. 1967 Oct;6(10):3307-14. doi: 10.1021/bi00862a041.
9
Properties of basic amino-acid residues. Nucleotide--poly(amino acid)interaction.碱性氨基酸残基的特性。核苷酸与聚氨基酸的相互作用。
Eur J Biochem. 1974 Jul 1;46(1):27-34. doi: 10.1111/j.1432-1033.1974.tb03593.x.
10
Aromatic amino acid-lysine copolymers. Conformation and specificity of nucleotide interaction.
Biochimie. 1974;56(1):53-60. doi: 10.1016/s0300-9084(74)80355-x.
Zotero 插件