Barb C Richard, Kraeling Robert R, Rampacek George B, Hausman Gary J
USDA/ARS, Animal Physiology Research Unit, Russell Research Center, Athens, GA 30604-5677, USA.
Reproduction. 2006 Jun;131(6):1127-35. doi: 10.1530/rep.1.01108.
Two experiments (EXP) were conducted in ovariectomized prepubertal gilts to test the hypothesis that neuropeptide Y (NPY) stimulates appetite and modulates LH and GH secretion, and that leptin modifies such acute effects of NPY on feeding behavior and LH and GH secretion. In EXP I, gilts received intracerebroventricular (ICV) injections of 0.9% saline (saline; n = 6), or 10 microg (n = 7), 50 microg (n = 5) or 100 microg (n = 7) NPY in saline and blood samples were collected. In EXP II, gilts received ICV injections of S (n = 4), or 50 microg leptin (n = 4), or 100 microg NPY (n = 4) or 100 microg NPY +50 microg leptin (n = 4) in saline, and feed intake was measured at 4, 20 and 44 h after feed presentation and blood samples collected. In EXP I, NPY suppressed LH secretion and the 100 microg dose stimulated GH secretion. In EXP II, NPY reversed the inhibitory effect of leptin on feed intake and suppressed LH secretion, but serum GH concentrations were unaffected. These results support the hypothesis that NPY modulates feed intake, and LH and GH secretion and may serve as a neural link between metabolic state and the reproductive and growth axis in the pig.
进行了两项实验（EXP），以测试以下假设：神经肽Y（NPY）刺激食欲并调节促黄体生成素（LH）和生长激素（GH）的分泌，并且瘦素会改变NPY对摄食行为以及LH和GH分泌的这种急性影响。在实验I中，给小母猪脑室内（ICV）注射0.9%生理盐水（生理盐水组；n = 6），或注射含10微克（n = 7）、50微克（n = 5）或100微克（n = 7）NPY的生理盐水，并采集血样。在实验II中，给小母猪脑室内注射生理盐水（n = 4）、或50微克瘦素（n = 4）、或100微克NPY（n = 4）或100微克NPY + 50微克瘦素（n = 4），在喂食后4小时、20小时和44小时测量采食量并采集血样。在实验I中，NPY抑制LH分泌，100微克剂量刺激GH分泌。在实验II中，NPY逆转了瘦素对采食量的抑制作用并抑制LH分泌，但血清GH浓度未受影响。这些结果支持了以下假设：NPY调节采食量、LH和GH分泌，并且可能是猪的代谢状态与生殖和生长轴之间的神经联系。
