State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Animal Sciences and Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China.
Genes (Basel). 2024 Jan 29;15(2):181. doi: 10.3390/genes15020181.
The egg-laying performance of Shan Ma ducks (Anas Platyrhynchos) is a crucial economic trait. Nevertheless, limited research has been conducted on the egg-laying performance of this species. We examined routine blood indicators and observed higher levels of metabolic and immune-related factors in the high-egg-production group compared with the low-egg-production group. Furthermore, we explored the ovarian transcriptome of both high- and low-egg-production groups of Shan Ma ducks using Illumina NovaSeq 6000 sequencing. A total of 1357 differentially expressed genes (DEGs) were identified, with 686 down-regulated and 671 up-regulated in the high-egg-production (HEP) ducks and low-egg-production (LEP) ducks. Several genes involved in the regulation of ovarian development, including neuropeptide Y (NPY), cell cycle protein-dependent kinase 1 (CDK1), and transcription factor 1 (E2F1), exhibited significant differential expressions at varying stages of egg production. Pathway functional analysis revealed that the DEGs were primarily associated with the steroid biosynthesis pathway, and the neuroactive ligand-receptor interaction pathway exhibited higher activity in the HEP group compared to the LEP group. This study offers valuable information about and novel insights into high egg production.
山麻鸭的产蛋性能是一个重要的经济性状。然而，目前对该品种的产蛋性能研究较少。我们检测了常规血液指标，发现高产蛋组鸭的代谢和免疫相关因子水平高于低产蛋组鸭。此外，我们还使用 Illumina NovaSeq 6000 测序技术对高产蛋组和低产蛋组山麻鸭的卵巢转录组进行了研究。共鉴定到 1357 个差异表达基因（DEGs），其中高产蛋（HEP）鸭和低产蛋（LEP）鸭中分别有 686 个下调和 671 个上调。一些参与卵巢发育调控的基因，如神经肽 Y（NPY）、细胞周期蛋白依赖性激酶 1（CDK1）和转录因子 1（E2F1），在产蛋不同阶段表现出显著的差异表达。通路功能分析表明，差异表达基因主要与甾体生物合成途径相关，神经活性配体-受体相互作用途径在高产蛋组鸭中比低产蛋组鸭更为活跃。本研究为高产蛋提供了有价值的信息和新的见解。
