Gutjahr C, Nick P
Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Freiburg im Breisgau.
Protoplasma. 2006 May;227(2-4):211-22. doi: 10.1007/s00709-005-0140-4. Epub 2006 May 3.
To find components which participate in gravitropic signal transmission, we screened different cell biological inhibitors for their effect on gravitropic bending of rice coleoptiles. Acrylamide, which is known to affect intermediate filaments in mammalian cells, strongly inhibited gravitropic bending at concentrations that did not inhibit growth of coleoptile segments. This inhibition was reversible. Investigating the acrylamide effect further, we found that it interferes with an event that occurs around 15 min after the onset of stimulation. We also observed that acrylamide inhibits polar indolyl-3-acetic acid transport. Furthermore, acrylamide efficiently eliminated microtubules, whereas actin filaments remained intact. To our knowledge this is the first report of effects of monoacrylamide in plant cells.
为了找到参与重力信号传导的成分，我们筛选了不同的细胞生物学抑制剂，以研究它们对水稻胚芽鞘重力弯曲的影响。已知丙烯酰胺会影响哺乳动物细胞中的中间丝，在不抑制胚芽鞘切段生长的浓度下，它能强烈抑制重力弯曲。这种抑制是可逆的。进一步研究丙烯酰胺的作用，我们发现它干扰了刺激开始后约15分钟发生的一个事件。我们还观察到丙烯酰胺抑制极性吲哚-3-乙酸运输。此外，丙烯酰胺能有效消除微管，而肌动蛋白丝保持完整。据我们所知，这是关于单丙烯酰胺对植物细胞作用的首次报道。
