Sekula Justyna, Everaert Joseph, Bohets Hugo, Vissers Bert, Pietraszkiewicz Marek, Pietraszkiewicz Oksana, Du Prez Filip, Vanhoutte Koen, Prus Piotr, Nagels Luc J
Chemistry Department, Antwerp University, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Anal Chem. 2006 Jun 1;78(11):3772-9. doi: 10.1021/ac060066y.
Capillary electrophoresis was coupled successfully and reliably to potentiometric sensors, which are based on an ionically conductive rubber phase coating, applied on a 250 microm diameter metal substrate. The membrane components included potassium tetrakis(p-chlorophenyl)borate (TCPB), bis(2-ethylhexyl)sebacate (DOS), and high molecular mass poly(vinyl chloride) (PVC). Potentiometry reveals a very sensitive CE detection mode, with sub-micromolar detection limits for amines and the randomly chosen drugs quinine, clozapine, cocaine, heroine, noscapine, papaverine, and ritodrine. The lowest detection limit, 1 x 10(-8) M injected concentration, was obtained for the quaternary ammonium compound tetrahexylammonium chloride. The more polar lower aliphatic amines and the biogenic amines dopamine, adrenaline, and cadaverine have much higher detection limits. The detection limits are log P dependent. Addition of a commercially available calixarene molecule or a synthetic macrocyclic amphiphilic receptor molecule to the electrode coatings enhanced the sensitivity respectively for the lower aliphatic amines and for the biogenic amines. A transpose of the Nikolskii-Eisenman-type function was suggested and used to convert the signal of the detector to a concentration-dependent signal.
毛细管电泳已成功且可靠地与电位传感器联用，该电位传感器基于涂覆在直径为250微米的金属基底上的离子导电橡胶相涂层。膜组件包括四（对氯苯基）硼酸钾（TCPB）、癸二酸二（2-乙基己基）酯（DOS）和高分子量聚氯乙烯（PVC）。电位分析法揭示了一种非常灵敏的毛细管电泳检测模式，对胺类以及随机选择的药物奎宁、氯氮平、可卡因、海洛因、那可丁、罂粟碱和利托君的检测限可达亚微摩尔级。对于季铵化合物氯化四己铵，获得了最低检测限，即注入浓度为1×10⁻⁸ M。极性更强的低级脂肪胺以及生物胺多巴胺、肾上腺素和尸胺的检测限要高得多。检测限与log P相关。向电极涂层中添加市售杯芳烃分子或合成大环两亲受体分子，分别提高了对低级脂肪胺和生物胺的灵敏度。提出并使用了Nikolskii-Eisenman型函数的变换形式，将检测器的信号转换为浓度相关信号。
