Simó Carolina, Moreno-Arribas M Victoria, Cifuentes Alejandro
Institute of Industrial Fermentations (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
J Chromatogr A. 2008 Jun 27;1195(1-2):150-6. doi: 10.1016/j.chroma.2008.05.004. Epub 2008 May 10.
In this work, two different capillary electrophoresis-mass spectrometry (CE-MS) methods, namely, capillary electrophoresis-ion-trap mass spectrometry (CE-IT-MS) and capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS), applied to analyze biogenic amines in wine samples are investigated. A group of five amines was selected as case study (namely, putrescine, cadaverine, histamine, phenylethylamine and tyramine) since they are the most frequently biogenic amines found in wines. The possibilities of both instruments in terms of sensitivity, selectivity and quantitation during the determination of the mentioned biogenic amines in wine samples was studied and their results corroborated by HPLC. After optimization of the analytical conditions, CE-IT-MS and CE-TOF-MS allowed the identification of biogenic amines in wines without any previous treatment except diluting 1:1 with water and filtering. Biogenic amines were determined in three red wines and one white wine showing, as expected, a higher concentration in red wines. Moreover, CE-IT-MS and CE-TOF-MS were compared regarding their capacity to detect other biogenic amines different to the selected ones in wine samples, showing CE-TOF-MS a much better capability (i.e., putrescine, cadaverine, histamine, phenylethylamine, tyramine, triptamine, spermidine, spermine, ethanolamine and isoamylamine were identified by CE-TOF-MS in a single analysis). Moreover, CE-TOF-MS allowed the quantitation of biogenic amines with limits of detection as low as 10 ng/mL, comparable to those obtained using HPLC with fluorescence detection, without any previous derivatization step and with analysis times fivefold faster (40 min by HPLC and 8 min by CE-TOF-MS).
在本研究中，对两种不同的毛细管电泳-质谱联用（CE-MS）方法，即毛细管电泳-离子阱质谱联用（CE-IT-MS）和毛细管电泳-飞行时间质谱联用（CE-TOF-MS）用于分析葡萄酒样品中的生物胺进行了研究。选择了一组五种胺作为案例研究对象（即腐胺、尸胺、组胺、苯乙胺和酪胺），因为它们是葡萄酒中最常见的生物胺。研究了这两种仪器在测定葡萄酒样品中上述生物胺时的灵敏度、选择性和定量能力，并通过高效液相色谱法（HPLC）对其结果进行了验证。在优化分析条件后，CE-IT-MS和CE-TOF-MS无需任何预处理，只需用水1:1稀释并过滤，就能鉴定葡萄酒中的生物胺。对三种红葡萄酒和一种白葡萄酒中的生物胺进行了测定，正如预期的那样，红葡萄酒中的浓度较高。此外，还比较了CE-IT-MS和CE-TOF-MS在检测葡萄酒样品中除选定生物胺之外的其他生物胺的能力，结果表明CE-TOF-MS的能力更强（即，CE-TOF-MS在一次分析中鉴定出了腐胺、尸胺、组胺、苯乙胺、酪胺、色胺、亚精胺、精胺、乙醇胺和异戊胺）。此外，CE-TOF-MS能够对生物胺进行定量，检测限低至10 ng/mL，与使用荧光检测的HPLC相当，无需任何预先衍生步骤，分析时间快五倍（HPLC为40分钟，CE-TOF-MS为8分钟）。
