Fox Keith R, Brown Tom
School of Biological Sciences, University of Southampton, Southampton, UK.
Q Rev Biophys. 2005 Nov;38(4):311-20. doi: 10.1017/S0033583506004197. Epub 2006 May 31.
Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored.
沃森-克里克碱基配对是一种自然的分子识别过程，已在分子生物学中得到应用，并在许多领域被广泛采用。一种可与正常碱基配对结合使用的核酸序列识别附加模式，将为核酸相互作用增添一个额外维度，并开辟许多新的应用。原则上，如果能开发出识别任何DNA序列的三链体方法，就能实现这一点。为此，已将修饰核苷掺入形成三链体的寡核苷酸（TFO）中，并用于在中性pH下以高选择性和亲和力识别混合序列DNA。持续的研究进展旨在提高TFO在高pH下的亲和力，并加快三链体的缔合动力学。目前正在探索三链体的多种应用。
