Peng Zuogang, Wang Jun, He Shunping
Laboratory of Fish Phylogenetics and Biogeography, Institute of Hydrobiology, Chinese Academy of Sciences, 7th Donghu South Road, Wuhan 430072, China.
Gene. 2006 Jul 19;376(2):290-7. doi: 10.1016/j.gene.2006.04.014. Epub 2006 May 3.
The complete sequence of the 16,539 nucleotide mitochondrial genome from the single species of the catfish family Cranoglanididae, the helmet catfish Cranoglanis bouderius, was determined using the long and accurate polymerase chain reaction (LA PCR) method. The nucleotide sequences of C. bouderius mitochondrial DNA have been compared with those of three other catfish species in the same order. The contents of the C. bouderius mitochondrial genome are 13 protein-coding genes, two ribosomal RNA and 22 transfer RNA genes, and a non-coding control region, the gene order of which is identical to that observed in most other vertebrates. Phylogenetic analyses for 13 otophysan fishes were performed using Bayesian method based on the concatenated mtDNA protein-coding gene sequence and the individual protein-coding gene sequence data set. The competing otophysan topologies were then tested by using the approximately unbiased test, the Kishino-Hasegawa test, and the Shimodaira-Hasegawa test. The results show that the grouping ((((Characiformes, Gymnotiformes), Siluriformes), Cypriniformes), outgroup) is the most likely but there is no significant difference between this one and the other alternative hypotheses. In addition, the phylogenetic placement of the family Cranoglanididae among siluriform families was also discussed.
利用长片段精确聚合酶链反应（LA PCR）方法，测定了鲶鱼科（Cranoglanididae）唯一物种——头盔鲶（Cranoglanis bouderius）的16539个核苷酸线粒体基因组的完整序列。将头盔鲶线粒体DNA的核苷酸序列与同一目下的其他三种鲶鱼的序列进行了比较。头盔鲶线粒体基因组包含13个蛋白质编码基因、两个核糖体RNA基因和22个转运RNA基因，以及一个非编码控制区，其基因顺序与大多数其他脊椎动物中观察到的相同。基于串联的线粒体DNA蛋白质编码基因序列和单个蛋白质编码基因序列数据集，采用贝叶斯方法对13种骨鳔鱼类进行了系统发育分析。然后使用近似无偏检验、木村-原田检验和岛田-原田检验对相互竞争的骨鳔鱼类拓扑结构进行了检验。结果表明，分组（(((脂鲤目, 电鳗目), 鲶形目), 鲤形目), 外类群）是最有可能的，但这一结果与其他替代假设之间没有显著差异另外，还讨论了头盔鲶科在鲶形目各科中的系统发育位置。
