Clode Peta L
Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, WA 6009, Australia.
J Struct Biol. 2006 Sep;155(3):505-11. doi: 10.1016/j.jsb.2006.04.004. Epub 2006 May 17.
Charge contrast imaging (CCI) is a dynamic phenomenon recently reported in insulating and semiconducting materials imaged with low vacuum or variable pressure scanning electron microscopes (SEM). Data presented in this paper illustrates that CCI can also be applied to biominerals and biological soft-tissues and that useful and unique structural information can be obtained from routine samples. Various resin-embedded samples were considered and example images from several different biomaterials are presented. Due to the diverse nature of samples that appear to exhibit charge contrast, this imaging technique has prospective application in a wide range of biological and biomedical research. This work represents the first application of CCI to biomaterials and in particular, highlights a new method for investigating the formation, structure and growth of biominerals.
电荷对比成像（CCI）是一种最近在使用低真空或可变压力扫描电子显微镜（SEM）成像的绝缘和半导体材料中报道的动态现象。本文呈现的数据表明，CCI也可应用于生物矿物和生物软组织，并且可以从常规样品中获得有用且独特的结构信息。我们考虑了各种树脂包埋的样品，并展示了几种不同生物材料的示例图像。由于出现电荷对比的样品性质多样，这种成像技术在广泛的生物学和生物医学研究中具有潜在应用。这项工作代表了CCI在生物材料中的首次应用，尤其突出了一种研究生物矿物形成、结构和生长的新方法。
